Poerschmann Juergen, Rauschen Stefan, Langer Uwe, Augustin Juergen, Górecki Tadeusz
Department of Environmental Technology, UFZ-Helmholtz Centre for Environmental Research, Permoserstrasse 15, D-04318 Leipzig, Germany.
J Agric Food Chem. 2008 Dec 24;56(24):11906-13. doi: 10.1021/jf8023694.
Bt-maize MON88017, its near-isogenic line DKC5143, and the two conventional varieties DK315 and Benicia were subjected to tetramethylammonium hydroxide (TMAH)-induced thermochemolysis to reveal molecular level lignin patterns. MON88017 is genetically modified to express the Cry3Bb1 protein aimed at the Western corn rootworm Diabrotica virgifera virgifera, a serious threat for European maize production. The results indicated that roots of the Bt-maize were characterized by a slightly enhanced total lignin content (by approximately 7%) compared to the near-isogenic line, whereas the molecular-based patterns, expressed by the relative fractions of p-hydroxyphenyl, guaiacyl, and syringyl breakdown products (P-, G-, and S-units, respectively) were virtually identical for both lines. No effects regarding either total lignin or molecular-based lignin patterns could be observed for leaves, indicating that biogenesis of lignin was not pleiotropically affected by the genetic modification. Significant differences for both total lignin and different lignin proxies existed between the conventional maize lines. Molecular level lignin analysis by means of TMAH-induced thermochemolysis is able to distinguish conventional maize varieties. Further work is necessary to evaluate lignin-related pleiotropic effects in genetically modified maize plants. The validation and application of a commonly accepted method for lignin analysis, capable of characterizing lignin at the molecular level, is a prerequisite.
将转Bt基因玉米MON88017、其近等基因系DKC5143以及两个常规品种DK315和贝尼西亚进行氢氧化四甲铵（TMAH）诱导的热化学降解，以揭示分子水平的木质素模式。MON88017经过基因改造，可表达针对西部玉米根萤叶甲的Cry3Bb1蛋白，这种害虫对欧洲玉米生产构成严重威胁。结果表明，与近等基因系相比，转Bt基因玉米根中的总木质素含量略有增加（约7%），而基于分子的模式，即对羟基苯基、愈创木基和紫丁香基分解产物（分别为P-、G-和S-单元）的相对比例，在两个品系中几乎相同。在叶片中未观察到总木质素或基于分子的木质素模式有任何影响，这表明木质素的生物合成并未受到基因改造的多效性影响。常规玉米品系之间在总木质素和不同木质素指标方面均存在显著差异。通过TMAH诱导的热化学降解进行分子水平的木质素分析能够区分常规玉米品种。有必要开展进一步的工作来评估转基因玉米植株中与木质素相关的多效性效应。验证并应用一种能够在分子水平上表征木质素的公认木质素分析方法是前提条件。
