Yamada Masato, Kurano Michihisa, Inatomi Satoshi, Taguchi Goro, Okazaki Mitsuo, Shimosaka Makoto
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Tokida, Ueda, Nagano, Japan.
FEMS Microbiol Lett. 2008 Dec;289(2):130-7. doi: 10.1111/j.1574-6968.2008.01361.x.
Fv-pda, a gene coding for chitin deacetylase (CDA), was isolated from the basidiomycete Flammulina velutipes by differential display targeted for genes specifically expressed during fruiting body development. The fv-pda ORF comprises 250 amino acid residues and is interrupted by 10 introns. The fv-pda cDNA was expressed in the yeast Pichia pastoris, and the resulting recombinant FV-PDA was used for enzymatic characterization. The recombinant FV-PDA catalyses deacetylation of N-acetyl-chitooligomers, from dimer to pentamer, glycol chitin and colloidal chitin. The fv-pda was specifically expressed through the entire stage of fruiting body development, and the transcript was abundant in stipes of mature fruiting bodies. These results suggest that CDA plays an important role in the process of fruiting of F. velutipes.
Fv-pda是一种编码几丁质脱乙酰酶（CDA）的基因，它是通过差异显示从担子菌金针菇中分离出来的，该差异显示针对的是在子实体发育过程中特异性表达的基因。fv-pda开放阅读框包含250个氨基酸残基，并被10个内含子中断。fv-pda cDNA在毕赤酵母中表达，所得重组FV-PDA用于酶学特性分析。重组FV-PDA催化N-乙酰壳寡糖（从二聚体到五聚体）、乙二醇几丁质和胶体几丁质的脱乙酰化反应。fv-pda在子实体发育的整个阶段特异性表达，且转录本在成熟子实体的菌柄中大量存在。这些结果表明CDA在金针菇的结实过程中起重要作用。
