苏云金芽孢杆菌和蜡样芽孢杆菌产生的溶血素的特性

Identity of hemolysins produced by Bacillus thuringiensis and Bacillus cereus.

作者信息

Honda T, Shiba A, Seo S, Yamamoto J, Matsuyama J, Miwatani T

机构信息

Research Institute for Microbial Diseases, Osaka University, Japan.

出版信息

FEMS Microbiol Lett. 1991 Apr 15;63(2-3):205-9. doi: 10.1016/0378-1097(91)90087-q.

DOI:10.1016/0378-1097(91)90087-q

PMID:1905656

Abstract

A hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis var. kurstaki HD-1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45-65%) and column chromatography using DEAE-cellulose, Sephadex G-75 and KB-002 (a hydroxyapatite column for fast protein liquid chromatography). A hemolysin (Bc-hemolysin) produced by B. cereus HG-6A was also purified by the same procedure. The purified Bt-hemolysin and Bc-hemolysin, both of which are thiol-activated hemolysins, were biologically, physicochemically and immunologically identical. These findings provide further evidence of the similarity of B. thuringiensis, which is being used as a biological insecticide, to B. cereus, a toxigenic organism of food poisoning.

摘要

由产生晶体毒素的苏云金芽孢杆菌库尔斯塔克变种HD-1产生的一种溶血素（Bt-溶血素），通过硫酸铵（45%-65%）连续处理以及使用DEAE-纤维素、葡聚糖G-75和KB-002（用于快速蛋白质液相色谱的羟基磷灰石柱）进行柱色谱法进行纯化。蜡样芽孢杆菌HG-6A产生的一种溶血素（Bc-溶血素）也通过相同程序进行纯化。纯化后的Bt-溶血素和Bc-溶血素均为硫醇激活型溶血素，在生物学、物理化学和免疫学上具有同一性。这些发现进一步证明了作为生物杀虫剂使用的苏云金芽孢杆菌与食物中毒产毒生物蜡样芽孢杆菌之间的相似性。