Czeczuga Bazyli, Czeczuga-Semeniuk Ewa, Semeniuk Adrianna
Department of General Biology, Medical University, Kilińiskiego 1, 15-089 Białystok, Poland.
Folia Biol (Krakow). 2005;53(3-4):109-14. doi: 10.3409/173491605775142756.
Column (CC), thin-layer (TLC), high-performance liquid (HPLC) and ion-exchange chromatography (IEC), were used to investigate corotenoid and carotenoprotein complexes in Asellus aquaticus specimens from the Narew river. The following carotenoids were found: alpha-carotene, beta-carotene, beta-cryptoxanthin, lutein, zeaxanthin, diadinoxanthin, mutatoxanthin, crustaxanthin, echinenone, hydroxyechinenone, phoenicoxanthin, canthaxanthin and astaxanthin. Astaxanthin (37.5%), canthaxanthin (21.4%) and phoenicoxanthin (12.3%) were found in the largest amounts. The total carotenoid content was 13.824 microg g(-1) of dry mass. Carotenoprotein complexes containing astaxanthin as the prosthetic group were purified from Asellus aquaticus. The carotenoprotein complexes belonged to the crustacyanins group as alpha- and gamma-crustacyanin. The protein forming the alpha-crustacyanin contained large amounts of such amino-acids as asparic acid, glutamic acid and leucine, whereas the protein of the gamma-crustacyanin contained primarily glutamic acid, glycine and lysine.
采用柱色谱（CC）、薄层色谱（TLC）、高效液相色谱（HPLC）和离子交换色谱（IEC），对来自纳雷夫河的普通等足虫样本中的类胡萝卜素和类胡萝卜素蛋白复合物进行了研究。发现了以下类胡萝卜素：α-胡萝卜素、β-胡萝卜素、β-隐黄质、叶黄素、玉米黄质、二海胆藻黄素、变藻黄素、虾青素、海胆酮、羟基海胆酮、凤仙黄质、角黄素和虾青素。其中虾青素含量最高（37.5%），其次是角黄素（21.4%）和凤仙黄质（12.3%）。总类胡萝卜素含量为每克干重13.824微克。从普通等足虫中纯化出了以虾青素作为辅基的类胡萝卜素蛋白复合物。这些类胡萝卜素蛋白复合物属于甲壳蓝蛋白组，即α-和γ-甲壳蓝蛋白。构成α-甲壳蓝蛋白的蛋白质含有大量天冬氨酸、谷氨酸和亮氨酸等氨基酸，而γ-甲壳蓝蛋白的蛋白质主要含有谷氨酸、甘氨酸和赖氨酸。
