Marangos Orestes, Misra Anil, Spencer Paulette, Bohaty Brenda, Katz J Lawrence
Civil, Environmental and Architectural Engineering Department, University of Kansas, 1530 W. 15th Street, Lawrence, KS 66045-7609, USA.
Acta Biomater. 2009 May;5(4):1338-48. doi: 10.1016/j.actbio.2008.10.023. Epub 2008 Nov 17.
Microscale elastic moduli, composition and density have rarely been determined at the same location for biological materials. In this paper, we have performed homotopic measurements to determine the physico-mechanical properties of a second primary molar specimen exhibiting sound and caries-affected regions. A microscale acoustic impedance map of a section through this sample was acquired using scanning acoustic microscopy (SAM). Scanning electron microscopy was then used to obtain mineral mass fraction of the same section using backscattered images. Careful calibration of each method was performed to reduce system effects and obtain accurate data. Resorption, demineralization and hypermineralization mechanisms were considered in order to derive relationships between measured mineral mass fraction and material mass density. As a result, microscale mass density was determined at the same lateral resolution and location as the SAM data. The mass density and the acoustic impedance were combined to find the microscale elastic modulus and study the relationship between microscale composition and mechanical properties.
对于生物材料，很少在同一位置同时测定微观弹性模量、成分和密度。在本文中，我们进行了同位测量，以确定一个同时具有健康区域和龋齿区域的第二恒磨牙样本的物理力学性能。使用扫描声学显微镜（SAM）获取了该样本切片的微观声阻抗图。然后使用扫描电子显微镜通过背散射图像获得同一切片的矿物质质量分数。对每种方法进行了仔细校准，以减少系统影响并获得准确数据。考虑了吸收、脱矿质和矿质过多机制，以推导所测矿物质质量分数与材料质量密度之间的关系。结果，在与SAM数据相同的横向分辨率和位置确定了微观质量密度。将质量密度和声阻抗相结合，以找到微观弹性模量，并研究微观成分与力学性能之间的关系。
