Choi Nack-Shick, Kim Bo-Hye, Park Chang-Su, Han Yun Jon, Lee Hye Won, Choi Jong Hyun, Lee Seung-Goo, Song Jae Jun
Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, Sinjeong-dong, Jeonbuk, Republic of Korea.
Anal Biochem. 2009 Mar 1;386(1):121-2. doi: 10.1016/j.ab.2008.11.007. Epub 2008 Nov 13.
We have developed a system to detect three hydrolytic enzymes (cellulase, lipase, and protease) using a single sodium dodecyl sulfate (SDS) gel and an electrotransfer system. After electrophoresis, proteins in the gel were transferred to three sandwiched substrate gels containing glycerol tributyrate, azo-carboxymethyl cellulose (Azo-CMC), and fibrin for detection of cellulase, lipase, and protease, respectively. We show that three cellulases (from a Paenibacillus sp. and two Bacillus sp. strains), one lipase (from a Staphylococcus sp.), and two proteases (from two Bacillus sp. strains) can be detected simultaneously with our zymogram system.
我们开发了一种系统，可使用单一的十二烷基硫酸钠（SDS）凝胶和电转印系统来检测三种水解酶（纤维素酶、脂肪酶和蛋白酶）。电泳后，凝胶中的蛋白质被转移到分别含有三丁酸甘油酯、偶氮羧甲基纤维素（Azo-CMC）和纤维蛋白的三种夹心底物凝胶上，以分别检测纤维素酶、脂肪酶和蛋白酶。我们的酶谱系统可以同时检测出三种纤维素酶（分别来自一株类芽孢杆菌属细菌和两株芽孢杆菌属细菌）、一种脂肪酶（来自一株葡萄球菌属细菌）和两种蛋白酶（分别来自两株芽孢杆菌属细菌）。
