The effect of sample storage on the reliability of thyroglobulin and thyroglobulin-antibody measurements.
作者信息
Locsei Zoltán, Toldy Erzsébet, Szabolcs István, Rácz Károly, Kovács Gábor L
机构信息
1st Department of Medicine, Markusovszky Teaching Hospital of County Vas, Szombathely, Hungary.
出版信息
Clin Biochem. 2009 Feb;42(3):225-8. doi: 10.1016/j.clinbiochem.2008.10.021. Epub 2008 Nov 11.
OBJECTIVES
To investigate how sample-storage alters thyroglobulin (Tg) and thyroglobulin-antibody (TgAb) immunoreactivity.
DESIGN AND METHODS
Tg and TgAb measurements at different storage-times and -temperatures.
RESULTS
48-h-storage at 4-10 degrees C (but not 8 h at room temperature) increased Tg and decreased TgAb immunoreactivity by maximum +23% and -16%. 4-weeks-storage at -17 to -20 degrees C decreased both Tg and TgAb by max. 21-31%.
CONCLUSIONS
Storage of samples at 4-10 degrees C for >24-48 h, or at -17 to -20 degrees C for >2-3 weeks significantly alters Tg and TgAb immunoreactivity.
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