Dewilde M, Strelkov S V, Rabijns A, Declerck P J
Katholieke Universiteit Leuven, Belgium.
J Struct Biol. 2009 Feb;165(2):126-32. doi: 10.1016/j.jsb.2008.11.001. Epub 2008 Nov 27.
Here we report the crystal structure of a stablilized plasminogen activator inhibitor-1 variant (PAI-1-N150H-K154T-Q301P-Q319L-M354I (PAI-1-stab)) that shows a cleavage within the reactive centre loop. The new structure is of superior quality compared to the previously determined structure of the cleaved PAI-1-A335P mutant. We present a detailed comparison of the two structures and also compare them with the structure of the active PAI-1-stab. The structural data give important insights into the working mechanism of PAI-1 and also explain the role of various stabilizing mutations.
在此，我们报道了一种稳定化的纤溶酶原激活物抑制剂-1变体（PAI-1-N150H-K154T-Q301P-Q319L-M354I，即PAI-1-stab）的晶体结构，该变体在反应中心环内发生了裂解。与先前测定的裂解型PAI-1-A335P突变体结构相比，新结构质量更优。我们对这两种结构进行了详细比较，并将它们与活性PAI-1-stab的结构进行了对比。这些结构数据为PAI-1的作用机制提供了重要见解，也解释了各种稳定化突变的作用。
