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牛瘤胃中甲酸四氢叶酸合成酶基因(fhs)的多样性,甲酸四氢叶酸合成酶是还原性产乙酸过程中的关键酶。

Diversity of the formyltetrahydrofolate synthetase gene (fhs), a key enzyme for reductive acetogenesis, in the bovine rumen.

作者信息

Matsui Hiroki, Kojima Noriko, Tajima Kiyoshi

机构信息

Graduate School of Bioresources, Mie University, Tsu, Mie, Japan.

出版信息

Biosci Biotechnol Biochem. 2008 Dec;72(12):3273-6. doi: 10.1271/bbb.70375. Epub 2008 Dec 7.

Abstract

A clone library of the partial formyltetrahydrofolate synthetase gene (fhs), a key enzyme in reductive acetogenesis, was constructed from the DNA of bovine rumen contents. Diverse sequences were recovered, the majority of which were clustered with the fhs of authentic acetogens. Low similarity values to known fhs were observed in all sequences, suggesting the presence of unknown acetogens.

摘要

从牛瘤胃内容物的DNA构建了部分甲酰四氢叶酸合成酶基因(fhs)的克隆文库,fhs是还原产乙酸过程中的关键酶。回收了多种序列,其中大多数与正宗产乙酸菌的fhs聚集在一起。在所有序列中均观察到与已知fhs的相似性值较低,这表明存在未知的产乙酸菌。

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