Wilton J C, Williams D E, Strain A J, Parslow R A, Chipman J K, Coleman R
School of Biochemistry, University of Birmingham, United Kingdom.
Hepatology. 1991 Jul;14(1):180-3. doi: 10.1002/hep.1840140129.
An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viability of more than 95%. Peak fractions were enriched in couplets to 84.5% +/- 2.5%. After elutriation, couplets retained the ability to secrete fluorescent cholephiles into sealed canalicular vacuoles. The preparation can now be used in hepatobiliary and hepatotoxicity studies not possible with preparations in which they are minor components.
通过离心淘析法富集了最初制备的含有约30%双核肝细胞的大鼠肝细胞。加载到淘析器上的双核肝细胞中，87%在转子速度为1100转/分钟、中等流速60至80毫升/分钟时被洗脱；在此范围内洗脱的细胞保持超过95%的活力。峰值级分中的双核肝细胞富集至84.5%±2.5%。淘析后，双核肝细胞保留了将荧光亲胆剂分泌到封闭胆小管泡中的能力。该制剂现在可用于肝胆和肝毒性研究，而对于以其为次要成分的制剂则无法进行此类研究。
