一种利用4-硝基喹啉-1-氧化物检测培养的正常和着色性干皮病淋巴母细胞系之间差异存活的改进方法。

An improved method for the detection of differential survival between normal and xeroderma pigmentosum lymphoblastoid cell lines in culture with 4-nitroquinoline-1-oxide.

作者信息

Kiyohara C, Hirohata T, Kuratsune M, Nagayama J

机构信息

Department of Public Health, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Mutat Res. 1991 Jul;249(1):111-7. doi: 10.1016/0027-5107(91)90137-d.

DOI:10.1016/0027-5107(91)90137-d

PMID:1906129

Abstract

The effects of the UV-mimetic chemical 4-nitroquinoline-1-oxide (4-NQO) upon cell lines heterozygous or homozygous for the recessive mutant xeroderma pigmentosum (XP) were investigated. Human lymphoblastoid cell lines, which were established from 4 XP homozygote patients (XPL15, XPL17, XPL19 and XPL20). 2 XP heterozygote individuals (XPPL17 and XPML17) and 58 normal individuals, were cultured in the presence of 4-NQO at doses of 0, 2, 4 and 8 x 10(-6) M. Then the total cell number was counted and the viability of the cells was measured by the dye exclusion method using trypan blue and a newly devised fluorometric method with fluorescein diacetate. Results showed that 4-NQO affected, in increasing order of impairment, the cell lines: normal less than XP heterozygote less than XP homozygote.

摘要

研究了紫外线模拟化学物质4-硝基喹啉-1-氧化物（4-NQO）对隐性突变型着色性干皮病（XP）杂合或纯合细胞系的影响。从4名XP纯合子患者（XPL15、XPL17、XPL19和XPL20）、2名XP杂合子个体（XPPL17和XPML17）以及58名正常个体建立的人淋巴母细胞系，在存在剂量为0、2、4和8×10⁻⁶ M的4-NQO的情况下进行培养。然后计算细胞总数，并使用台盼蓝通过染料排斥法和新设计的用荧光素二乙酸酯的荧光测定法测量细胞活力。结果表明，4-NQO以损伤程度递增的顺序影响细胞系：正常细胞系＜XP杂合子细胞系＜XP纯合子细胞系。