Pantzartzi Chrysoula N, Kourtidis Antonis, Drosopoulou Elena, Yiangou Minas, Scouras Zacharias G
Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, Thessaloniki, GR-54124 Greece.
Gene. 2009 Feb 15;431(1-2):47-54. doi: 10.1016/j.gene.2008.10.028. Epub 2008 Nov 13.
The commercially important marine bivalve Mytilus galloprovincialis (Mediterranean mussel) is considered a valuable bioindicator, due to its exposure to various pollutants and extreme environmental conditions. Environmental responsive genes, such as the hsp90s, protect the structure and function of cells and accomplish a significant task in cellular homeostasis. To study the hsp90s in M. galloprovincialis a genomic library was screened and two hsp90s were isolated. Sequence analysis revealed that the two genes exhibit great similarities in both the 5' non-coding and the coding region but differ in the 3' non-coding region, as well as in three introns, due to the presence of repeated sequences. Few synonymous substitutions in the coding region of the genes result to an identical predicted polypeptide, which belongs to the cytoplasmic HSP90 subfamily. The 5' non-coding region contains a non-translated exon and multiple binding sites for various transcription factors. The presence of a p53 binding site in the promoter of the isolated genes raises questions about the possible implication of hsp90s in the molluscan leukemia.
具有商业重要性的海洋双壳贝类——地中海贻贝（Mytilus galloprovincialis），由于其暴露于各种污染物和极端环境条件下，被视为一种有价值的生物指示物。环境响应基因，如热休克蛋白90（hsp90s），可保护细胞的结构和功能，并在细胞内稳态中发挥重要作用。为了研究地中海贻贝中的hsp90s，对一个基因组文库进行了筛选，并分离出两个hsp90s。序列分析表明，这两个基因在5'非编码区和编码区都表现出高度相似性，但在3'非编码区以及三个内含子中存在差异，这是由于存在重复序列。基因编码区中很少的同义替换导致预测的多肽相同，该多肽属于细胞质HSP90亚家族。5'非编码区包含一个非翻译外显子和多个不同转录因子的结合位点。分离基因启动子中存在p53结合位点，这引发了关于hsp90s在软体动物白血病中可能作用的疑问。
