Fundación IVI, Instituto Universitario IVI, University of Valencia, C/Guadassuar 1 bajo, 46015 Valencia, Spain.
Fertil Steril. 2010 Feb;93(3):774-782.e1. doi: 10.1016/j.fertnstert.2008.10.019. Epub 2008 Dec 4.
To compare embryologic and clinical outcomes in terms of preimplantation development, implantation, pregnancy rates, and secretome profile of implanted blastocysts from the preimplantation genetic diagnosis program grown in sequential versus endometrial epithelial cell (EEC) coculture system.
Retrospective clinical study and prospective experimental study.
In vitro fertilization clinical unit and university research laboratory.
INTERVENTION(S): Blastomere biopsy, embryo culture, blastocyst transfer, and protein analysis of the media conditioned from implanted embryos obtained from coculture and sequential systems.
MAIN OUTCOME MEASURE(S): Clinical study: blastocyst, implantation, and gestation rates in own and donated oocytes. Experimental study: differential protein analysis of implanted embryos grown in coculture system versus sequential system.
RESULT(S): Of the 12,377 embryos analyzed, the blastocyst rates were 56.0% versus 45.9% in the coculture versus the sequential system, respectively, with own oocytes. With ovum donation, the rates were 70.5% versus 56.4%, respectively. Reproductive outcomes in terms of pregnancy rates (39.1% vs. 27.5%) and implantation rates (33.3% vs. 20.9%,) were statistically higher in EEC coculture versus sequential media. Furthermore, the protein profile of the EEC coculture versus the sequential system was obtained. Interleukin-6 (IL-6) was the most secreted protein by the EEC culture. Further ELISA experiments showed that the IL-6 present in the sequential medium diminished in implanted blastocysts.
CONCLUSION(S): The coculture system favors blastocyst development and implantation rates, given the contribution of the factors secreted by endometrial epithelial cells, such as IL-6.
比较胚胎植入前遗传学诊断（PGD）方案中胚胎在序贯培养和子宫内膜上皮细胞（EEC）共培养系统中的发育、植入、妊娠率以及囊胚分泌组特征。
回顾性临床研究和前瞻性实验研究。
体外受精临床单位和大学研究实验室。
卵裂球活检、胚胎培养、囊胚移植和共培养与序贯系统中植入胚胎条件培养基的蛋白质分析。
临床研究：自身和捐赠卵的囊胚、种植和妊娠率。实验研究：共培养系统与序贯系统中植入囊胚的差异蛋白分析。
在分析的 12377 个胚胎中，序贯培养系统中的囊胚率分别为 56.0%和 45.9%，而 EEC 共培养系统中的囊胚率分别为 70.5%和 56.4%，自身卵。在捐卵的情况下，妊娠率（39.1%vs.27.5%）和种植率（33.3%vs.20.9%）分别为 EEC 共培养高于序贯培养。此外，还获得了 EEC 共培养与序贯系统的蛋白质谱。白细胞介素 6（IL-6）是 EEC 培养分泌最多的蛋白。进一步的 ELISA 实验表明，序贯培养基中存在的 IL-6 在植入囊胚中减少。
EEC 共培养系统有利于囊胚发育和种植率，因为子宫内膜上皮细胞分泌的因子，如 IL-6，发挥了作用。
