Tiutiaev P Iu
Tsitologiia. 2008;50(10):907-10.
For a long period of time two factors have impeded investigations of myxosporidia physiology: the small size of their nuclei and the absence of data about existence of condensed chromosomes in these organisms. Significant progress in biological research methods provides new approaches to investigations of Myxozoa chromatin and chromosomal apparatus. Using confocal scanning microscopy chromosomes in the generative cell nuclei of Zschokkela nova (Klokacewa, 1914) were revealed and described in this work for the first time. It has been shown that their karyotype consists of six chromosomes (three pairs). Two pairs of chromosomes have an oblong rod-shaped form. Chromosomes of the third pair have a boomerang form (with a bend in the middle of the chromosomes body and with a thickening on one of the ends). Lengths of chromosomes: 5 microm (1st pair), 4.8 microm (2nd pair) and 3 microm (3rd pair). The culture of plasmodium cells Myxobolus pseudodispar (Gorbunova, 1936) was received.
长期以来，有两个因素阻碍了对粘孢子虫生理学的研究：其细胞核体积小，且缺乏关于这些生物中存在浓缩染色体的数据。生物学研究方法的重大进展为研究粘体动物染色质和染色体装置提供了新途径。在这项工作中，首次利用共聚焦扫描显微镜揭示并描述了新态茨氏虫（Zschokkela nova，克洛卡采娃，1914）生殖细胞核中的染色体。结果表明，其核型由六条染色体（三对）组成。两对染色体呈长方形棒状。第三对染色体呈回飞棒状（染色体体中部有一个弯曲，一端有增厚）。染色体长度：5微米（第一对）、4.8微米（第二对）和3微米（第三对）。获得了伪异形碘泡虫（Myxobolus pseudodispar，戈尔布诺娃，1936）疟原虫细胞培养物。
