Moby V, Kadi A, de Isla N, Stoltz J F, Menu P
Groupe Mécanique et Ingénierie Cellulaire et Tissulaire, CNRS 7563, Faculté de Médecine, Nancy Université-UHP, 54505 Vandoeuvre-lès-Nancy, France.
Biomed Mater Eng. 2008;18(4-5):199-204.
In tissue engineering, surface characteristics of a biomaterial are one of most important factors determining the compatibility with the environment. They influence attachment and growth of cells onto the material. In many cases, the surface should to be modified and engineered in the desired direction. The modification of non-adhesive surfaces with polyelectrolyte multilayer films (PMF) was recently depicted as a powerful technique to promote the growth of different cell lines. In this study, we evaluated the possible use of two different PMF as surface modification for the culture of mesenchymal stem cells (MSC). We used two types of PMF which differed by the nature of the initial anchoring layer which was poly(ethylenimine) (PEI) or poly(allylamine hydrochloride) (PAH). This initial polyelectrolytes adsorption was followed by the alternated deposition of poly(sodium 4-styrenesulfonate) (PSS) and (PAH) in order to obtain a PEI-(PSS-PAH)(3) film or a PAH-(PSS-PAH)(3) film. In order to control the behaviour of MSC, the cell viability was evaluated by Alamar Blue assay and the actin cytoskeleton was labelled and visualised in a confocal microscope. The behaviour of cells on the two PMF was compared to cells cultivated on surfaces treated with fibronectin. The results showed that PAH-(PSS-PAH)(3) PMF improve the growth of cells, inducing a higher cell viability compared to PEI-(PSS-PAH)(3) PMF and fibronectin at 2, 3 and 7 days of culture. Moreover, those cells showed a well-organized actin cytoskeleton. In conclusion, PAH-(PSS-PAH)(3) polyelectrolyte multilayer film seems to constitute an excellent material for MSC seeding.
在组织工程中，生物材料的表面特性是决定其与周围环境相容性的最重要因素之一。它们会影响细胞在材料上的附着和生长。在许多情况下，需要对表面进行改性并朝着期望的方向进行工程处理。最近，用聚电解质多层膜（PMF）对非粘性表面进行改性被描述为一种促进不同细胞系生长的强大技术。在本研究中，我们评估了两种不同的PMF作为间充质干细胞（MSC）培养表面改性材料的可能性。我们使用了两种类型的PMF，它们的初始锚定层性质不同，分别是聚乙烯亚胺（PEI）或聚烯丙胺盐酸盐（PAH）。在这种初始聚电解质吸附之后，交替沉积聚（4-苯乙烯磺酸钠）（PSS）和（PAH），以获得PEI-(PSS-PAH)₃膜或PAH-(PSS-PAH)₃膜。为了控制MSC的行为，通过Alamar Blue检测评估细胞活力，并在共聚焦显微镜下对肌动蛋白细胞骨架进行标记和观察。将两种PMF上细胞的行为与在纤连蛋白处理过的表面上培养的细胞进行比较。结果表明，在培养2天、3天和7天时，PAH-(PSS-PAH)₃ PMF能促进细胞生长，与PEI-(PSS-PAH)₃ PMF和纤连蛋白相比，能诱导更高的细胞活力。此外，这些细胞显示出组织良好的肌动蛋白细胞骨架。总之，PAH-(PSS-PAH)₃聚电解质多层膜似乎是用于MSC接种的优良材料。
