[中国非肥胖和肥胖人群中GNB3基因825C/T多态性分析]

Wang Xiaosu, Bai Huai, Fan Ping, Liu Rui, Liu Yu, Liu Bingwen

Unit of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China.

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Dec;25(6):670-4.

OBJECTIVE

To investigate the G-protein beta 3 subunit (GNB3) gene 825C/T polymorphism and its relationship to obesity in Chinese population.

METHODS

Three hundred and ninety nine subjects (270 non-obese and 129 obese individuals) from a population of Chinese Han nationality in Chengdu area were studied using PCR-restriction fragment length polymorphisms (PCR-RFLPs). Serum lipids were measured by enzymatic kits and apolipoproteins A I, A II, B100, C II, C III and E were measured by RID kits.

RESULTS

The frequencies of C and T alleles at the 825C/T site in obese and non-obese groups were 0.531 and 0.469, and 0.528 and 0.472, respectively. It showed no significant difference in both genotypes and allele frequencies between the non-obese and obese groups (P> 0.05). The frequency of T allele at 825C/T site in GNB3 gene in the population (0.471) was significantly higher than that of German white (0.319), lower than that of African black (0.788), and similar to that of Japanese (0.487). In the non-obese group, subjects with genotype TT had higher serum triglyceride(TG) concentrations than those with genotype CT (P< 0.05). In the obese group, subjects with genotype CC had lower serum high-density lipoprotein cholesterol(HDL-C) levels than those with CT genotype (P< 0.05). Similar results were only observed in non-obese male and obese female subgroups, respectively, when male and female subgroups were further separated in the two groups. In addition, non-obese males with genotype TT and obese females with genotype CC had lower HDL-C and higher apoA I levels than those with genotype CT, respectively. Obese males with genotype TT had higher apoA I levels than those with genotype CC.

CONCLUSION

The 825C/T polymorphism in the GNB3 gene was not associated with obesity in Chinese Han population of Chengdu area. However, it may be associated with serum triglyceride, HDL-C and apoAI levels, with some gender-specific effect, in this population.

目的

研究中国人群中G蛋白β3亚基（GNB3）基因825C/T多态性及其与肥胖的关系。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLPs）技术，对成都地区399名中国汉族人群（270名非肥胖者和129名肥胖者）进行研究。用酶试剂盒测定血脂，用免疫比浊法试剂盒测定载脂蛋白A I、A II、B100、C II、C III和E。

结果

肥胖组和非肥胖组825C/T位点C和T等位基因频率分别为0.531和0.469，以及0.528和0.472。非肥胖组和肥胖组的基因型和等位基因频率均无显著差异（P>0.05）。该人群GNB3基因825C/T位点T等位基因频率（0.471）显著高于德国白人（0.319），低于非洲黑人（0.788），与日本人（0.487）相似。在非肥胖组中，基因型为TT的受试者血清甘油三酯（TG）浓度高于基因型为CT的受试者（P<0.05）。在肥胖组中，基因型为CC的受试者血清高密度脂蛋白胆固醇（HDL-C）水平低于基因型为CT的受试者（P<0.05）。当两组进一步分为男性和女性亚组时，仅在非肥胖男性和肥胖女性亚组中分别观察到类似结果。此外，基因型为TT的非肥胖男性和基因型为CC的肥胖女性的HDL-C水平较低，而载脂蛋白A I水平分别高于基因型为CT的受试者。基因型为TT的肥胖男性的载脂蛋白A I水平高于基因型为CC的男性。