Amorim J H, Macena T N S, Lacerda G V, Rezende R P, Dias J C T, Brendel M, Cascardo J C M
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Genet Mol Res. 2008;7(4):1226-32. doi: 10.4238/vol7-4gmr509.
The need for the prospecting for and identification of new biomolecules is a reality. Molecular techniques allow access to the metabolic potential of microorganisms via the isolation of DNA from environmental samples, i.e., without the application of microbial culture techniques. With its great biological diversity, the Atlantic Rainforest biome has a soil rich in organic matter, some components of which interfere negatively in the reactions necessary for the exploitation of its biotechnological potential. Here, we describe a protocol for the optimization of the treatment of soil samples before DNA extraction. The new methodology gives higher yield and quality of extracted DNA as compared with pre-existing techniques, facilitating the amplification and digestion of environmental DNA, and thus allows optimal exploitation of the genetic potential of the Atlantic Rainforest biome.
寻找和鉴定新生物分子的需求是切实存在的。分子技术能够通过从环境样本中分离DNA来挖掘微生物的代谢潜力，也就是说无需应用微生物培养技术。大西洋雨林生物群落具有丰富的生物多样性，其土壤富含有机质，其中一些成分会对开发其生物技术潜力所需的反应产生负面影响。在此，我们描述了一种在DNA提取前优化土壤样本处理的方案。与现有技术相比，新方法能提高提取DNA的产量和质量，便于环境DNA的扩增和消化，从而实现对大西洋雨林生物群落遗传潜力的最佳利用。
