Storrie Brian, Starr Tregei, Forsten-Williams Kimberly
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Methods Mol Biol. 2008;457:179-92. doi: 10.1007/978-1-59745-261-8_13.
With current light microscopy and laboratory-level computational capability, many questions in organelle assembly and membrane trafficking that were once treated in a qualitative manner can now be treated quantitatively. We present here an overview of the principles involved in doing quantitative fluorescence microscopy. We illustrate these with examples drawn from our work with the Golgi apparatus and endosomes in cultured mammalian cells. The principles themselves can be applied to any system.
借助当前的光学显微镜和实验室级别的计算能力，许多曾经以定性方式处理的细胞器组装和膜运输问题现在可以进行定量处理。我们在此概述进行定量荧光显微镜检查所涉及的原理。我们用从我们对培养的哺乳动物细胞中的高尔基体和内体的研究中得出的例子来说明这些原理。这些原理本身可应用于任何系统。
