Kuroiwa T, Okauchi M, Hua Y, Schallert T, Keep R F, Xi G
Clinical Laboratory, Namegata District General Hospital, Namegata, Ibaraki, Japan.
Acta Neurochir Suppl. 2008;105:127-30. doi: 10.1007/978-3-211-09469-3_26.
We examined the time course of neurological deficits in gerbils after an intracerebral hemorrhage (ICH) induced by autologous blood infusion and examined its correlation with the severity of perihematomal edema. Mongolian gerbils (n = 15) were subjected to stereotaxic autologous blood infusion (30 or 60 microL) into the left caudate nucleus. Corner-turn and forelimb-placing tests were performed before, and 1 and 3 days after ICH. Perihematomal water content was measured by tissue gravimetry. Gerbils developed neurological deficits and perihematomal edema at day 1 after ICH. Both neurological deficits and perihematomal edema were significantly greater in animals with 60 microL blood infusion compared to the 30 microL infusion group, and both neurological deficits and edema were also greater at 3 days compared to 1 day after ICH. The severity of neurological deficits paralleled the degree of perihematomal edema. We conclude that the Mongolian gerbil is a suitable model for studies on the behavioral effects of ICH.
我们研究了自体血注入诱导的脑出血（ICH）后沙鼠神经功能缺损的时间进程，并研究了其与血肿周围水肿严重程度的相关性。将15只蒙古沙鼠通过立体定向将自体血（30或60微升）注入左侧尾状核。在脑出血前、脑出血后1天和3天进行转角和前肢放置试验。通过组织重量法测量血肿周围含水量。沙鼠在脑出血后第1天出现神经功能缺损和血肿周围水肿。与注入30微升血液的动物相比，注入60微升血液的动物的神经功能缺损和血肿周围水肿均明显更严重，并且与脑出血后1天相比，在3天时神经功能缺损和水肿也更严重。神经功能缺损的严重程度与血肿周围水肿程度平行。我们得出结论，蒙古沙鼠是研究脑出血行为影响的合适模型。
