利用非洲爪蟾卵提取物分级分离进行体外细胞核组装。
In vitro nuclear assembly using fractionated Xenopus egg extracts.
作者信息
Cross Marie, Powers Maureen
机构信息
Department of Cell Biology, Emory University, USA.
出版信息
J Vis Exp. 2008 Sep 2(18):908. doi: 10.3791/908.
Abstract
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
摘要
核膜组装是细胞分裂周期中的一个重要步骤；通过将非洲爪蟾精子染色质、胞质溶胶和轻膜组分相结合，这个过程可以在试管中重现。完整的细胞核形成，包括带有孔复合体的核膜，并且这些重构的细胞核能够进行正常的核过程。
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