Reina J, Blanco I, Pericas C N, Morales C, Alomar P
Servicio de Microbiología, Hospital Son Dureta, Palma de Mallorca.
Enferm Infecc Microbiol Clin. 1991 Feb;9(2):76-81.
Phenotypic characteristics of 100 strains pertaining to the group of mesophilic aeromonas isolated in feces of patients with diarrhea (23 A. hydrophila, 34 A. sobria, 19 A. caviae, and 24 considered atypical because produced a the negative esculin reaction and a positive gas formation from glucose [TSI]). The percentages obtained in the different biochemical tests support the hypothesis that in this group there is a taxonomic complexity. We observed variations in the following tests: LDC, arabinose, Voges-Proskauser, lactose, and motility and hemolytic activity. We compared manual and automatic procedures in detecting esculinase and beta-galactosidase activity (ONPG). The study of constitutional enzymatic activity by means of API ZYM system can not be used to differentiate the distinct species although the enzyme beta-glucosidase is detected preferentially in A. hydrophila.
从腹泻患者粪便中分离出的100株嗜温气单胞菌的表型特征(23株嗜水气单胞菌、34株温和气单胞菌、19株豚鼠气单胞菌,以及24株因产生阴性七叶苷反应和葡萄糖产气阳性[三糖铁琼脂]而被视为非典型菌株)。在不同生化试验中获得的百分比支持了该菌群存在分类学复杂性这一假说。我们在以下试验中观察到了差异:赖氨酸脱羧酶试验、阿拉伯糖试验、Voges-Proskauer试验、乳糖试验以及运动性和溶血活性试验。我们比较了手动和自动检测七叶苷酶和β-半乳糖苷酶活性(邻硝基苯-β-D-半乳糖苷)的方法。尽管在嗜水气单胞菌中优先检测到β-葡萄糖苷酶,但通过API ZYM系统研究组成酶活性并不能用于区分不同的菌种。