Shin Chol, Kim Jin K, Kim Je H, Jung Ki H, Cho Kyung J, Lee Chang K, Lee Seung G
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Korea University, Seoul, Korea.
Psychiatry Clin Neurosci. 2008 Dec;62(6):721-7. doi: 10.1111/j.1440-1819.2008.01876.x.
Blood concentrations of cell-free DNA, which is considered to be released during apoptosis, are elevated under some pathological conditions such as cardiovascular disease and cancer. The association between obstructive sleep apnea (OSA) and cell-free DNA concentrations has not been reported so far. The purpose of the present study was to examine the association between OSA and plasma DNA concentrations.
A case-control study was conducted using a total of 164 men aged 39-67 years, who were free of coronary heart disease and cancer. Laboratory-based overnight polysomnography was performed for all participants.
On the basis of polysomnography, patients with an apnea-hypopnea index (AHI) = 5-30 events/h were defined as having mild-moderate OSA (n = 33) and those with >30 events/h were defined as having severe OSA (n = 49). All 82 controls had AHI < 5 events/h. Plasma DNA concentrations from all participants were analyzed for the beta-globin gene using fluorescence-based real-time polymerase chain reaction. Patients with severe OSA had significantly higher plasma DNA concentrations than persons with mild-moderate OSA and those without OSA (P < 0.05). AHI was significantly associated with body mass index (P < 0.001), hypertension (P < 0.001), and plasma DNA concentration (P < 0.05).
After taking into account hypertension and other potential risk factors, persons with high plasma DNA concentrations (>8 microg/L) had approximately fourfold higher odds of OSA than those with low DNA levels. Further data are warranted to confirm the association for men and to evaluate the association for women.
游离DNA的血药浓度在诸如心血管疾病和癌症等一些病理状况下会升高，其被认为是在细胞凋亡过程中释放出来的。迄今为止，阻塞性睡眠呼吸暂停（OSA）与游离DNA浓度之间的关联尚未见报道。本研究的目的是检验OSA与血浆DNA浓度之间的关联。
对总共164名年龄在39至67岁之间、无冠心病和癌症的男性进行了一项病例对照研究。对所有参与者进行了基于实验室的夜间多导睡眠图监测。
根据多导睡眠图，呼吸暂停低通气指数（AHI）=5-30次/小时的患者被定义为患有轻度至中度OSA（n=33），而AHI>30次/小时的患者被定义为患有重度OSA（n=49）。所有82名对照者的AHI<5次/小时。使用基于荧光的实时聚合酶链反应对所有参与者的血浆DNA浓度进行β-珠蛋白基因分析。重度OSA患者的血浆DNA浓度显著高于轻度至中度OSA患者和无OSA患者（P<0.05）。AHI与体重指数（P<0.001）、高血压（P<0.001）和血浆DNA浓度（P<0.05）显著相关。
在考虑高血压和其他潜在危险因素后，血浆DNA浓度高（>8μg/L）的人患OSA的几率比DNA水平低的人高出约四倍。需要进一步的数据来证实男性之间的关联，并评估女性之间的关联。
