硫酸二乙酯（DES）和叠氮化钠（SA）对蚕豆（豆科）诱导的有丝分裂畸变的比较分析。

Comparative analysis of mitotic aberrations induced by diethyl sulphate (DES) and sodium azide (SA) in Vicia faba L. (Fabaceae).

作者信息

Bhat Tariq Ahmad, Sharma Monika, Anis M

机构信息

Department of Botany, Cytogenetics and Mutation Breeding Research Laboratory, Aligarh Muslim University, Aligarh-202 002, India.

出版信息

Pak J Biol Sci. 2007 Mar 1;10(5):783-7. doi: 10.3923/pjbs.2007.783.787.

DOI:10.3923/pjbs.2007.783.787

PMID:19069864

Abstract

The present investigation provides a comparative account of different concentrations (0.01, 0.02, 0.03, 0.04, 0.05 and 0.06%) of diethylsulphate (DES) and Sodium Azide (SA) on mitotic aberrations, seed germination, seedling survival, plant height and mitotic index in Vicia faba L. variety major. The control plants were normal while as treated ones showed significant alterations. The mutagens caused dose dependent decrease in seed germination, seedling survival, plant height and mitotic index. All the parameters were found negatively affected and were positively correlated with mutagenic concentrations. The cytological study revealed various types of mitotic aberrations, among them the dominant were fragments, stickiness, precocious separation, c-metaphase, ring chromosomes, unequal separation, laggards, bridges, micronuclei, disturbed anaphase etc. Stickiness and fragments were more frequent as compared to other types.

摘要

本研究对不同浓度（0.01%、0.02%、0.03%、0.04%、0.05%和0.06%）的硫酸二乙酯（DES）和叠氮化钠（SA）对蚕豆（Vicia faba L.品种major）有丝分裂畸变、种子萌发、幼苗存活、株高和有丝分裂指数的影响进行了比较。对照植株正常，而处理过的植株表现出显著变化。诱变剂导致种子萌发、幼苗存活、株高和有丝分裂指数呈剂量依赖性下降。所有参数均受到负面影响，且与诱变剂浓度呈正相关。细胞学研究揭示了各种类型的有丝分裂畸变，其中主要的有片段、粘连、早熟分离、C中期、环状染色体、不等分离、落后染色体、桥、微核、后期紊乱等。与其他类型相比，粘连和片段更为常见。