Qian Xiao-Wei
School of Life and Environmental Science, Wenzhou Normal College, Wenzhou 325003, China.
J Zhejiang Univ Sci. 2004 Dec;5(12):1570-6. doi: 10.1631/jzus.2004.1570.
In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO(3)) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus aberration rate and chromosome aberration rate of Vicia faba root tip cells. The results showed that the effects of CrO(3) concentration on the mitotic indexes were complicated. CrO(3) increases the micronucleus aberration rate of Vicia faba root tip cells. It was found that within certain range of CrO(3) concentration the micronucleus aberration rate increased systematically with increased concentration of CrO(3), but that the micronucleus aberration rate decreased at higher level of CrO(3) and that CrO(3) also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO(3). We concluded that CrO(3) has significant mutagenic effect on Vicia faba root tip cells.
在这项关于不同浓度三氧化铬（CrO₃）对蚕豆根尖诱变效应的研究中，采用微核试验和染色体畸变试验来测定蚕豆根尖细胞的有丝分裂指数、微核畸变率和染色体畸变率。结果表明，CrO₃浓度对有丝分裂指数的影响较为复杂。CrO₃会增加蚕豆根尖细胞的微核畸变率。发现在CrO₃浓度的一定范围内，微核畸变率随CrO₃浓度的增加而系统性升高，但在较高CrO₃水平时微核畸变率下降，并且CrO₃还会导致各种类型的染色体畸变，其发生率随CrO₃浓度的增加而系统性升高。我们得出结论，CrO₃对蚕豆根尖细胞具有显著的诱变作用。
