Sinsiri N, Laohasiriwong S
Department of Agricultural Technology, Mahasarakham University, Mahasarakham 44000, Thailand.
Pak J Biol Sci. 2007 Jan 1;10(1):65-71. doi: 10.3923/pjbs.2007.65.71.
This experiment was carried out in the dry season (February-April, 2004) at the Experimental Farm, Mahasarakham University, Northeast Thailand to investigate the effect of different rates of indole-3-acitic acid (IAA) growth regulator in inducing root formation of detached leaves of MSU1, KVC#7 and IT84E-1-108 cowpea cultivars under tunnel conditions. The experiment was laid in a strip plot design with four replications and each replication was duplicated four times for four sampling periods. IAA levels used were 0, 250, 500, 750, 1,000, 1,500 and 2,000 mg L(-1) of distilled water, thus the experiment consisted of 21 treatments. The results showed that root length, number of both roots and root hairs were highly affected by IAA treatments and the best IAA level was found with level 3 (500 mg L(-1)). The effects due to IAA levels and cultivars were highly significant and the effects due to an interaction between factors A (cultivars) and B (IAA levels), in most cases, were highly significant. Detached leaf technique with the use of IAA growth regulator could be considered as one of the most appropriate techniques for the preparation of plant samples for the test on disease resistance of cowpea cultivars.
本实验于2004年2月至4月的旱季，在泰国东北部玛哈沙拉堪大学的实验农场进行，目的是研究在隧道条件下，不同浓度的吲哚 - 3 - 乙酸（IAA）生长调节剂对MSU1、KVC#7和IT84E - 1 - 108豇豆品种离体叶片生根的影响。实验采用条区设计，重复4次，每个重复在4个采样期内重复4次。使用的IAA浓度分别为0、250、500、750、1000、1500和2000 mg L(-1)的蒸馏水，因此实验共有21个处理。结果表明，根长、根数和根毛数受IAA处理的影响很大，最佳IAA浓度为3（500 mg L(-1)）。IAA浓度和品种的影响极显著，在大多数情况下，因素A（品种）和B（IAA浓度）之间的交互作用影响也极显著。使用IAA生长调节剂的离体叶片技术可被视为豇豆品种抗病性测试植物样本制备的最合适技术之一。
