Tiede Stephan, Ernst Nancy, Bayat Ardeshir, Paus Ralf, Tronnier Volker, Zechel Christina
Allergology and Venerology, Department of Dermatology, University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Ann Anat. 2009 Jan;191(1):33-44. doi: 10.1016/j.aanat.2008.10.001. Epub 2008 Nov 8.
Numerous tissue niches in the human body, such as skin, are now recognized to harbour adult stem cells. In this study, we analyze multipotent human dermis-derived progenitor cell populations, isolated and propagated from mechanically and enzymatically processed adult scalp skin. The populations encompass Nestin-positive and -negative cells, which may serve as a convenient and abundant source for various therapeutic applications in regenerative medicine. Here, we show that these cultures exhibit a strong tendency to differentiate into mesodermal derivatives, particularly myofibroblasts, when maintained in media containing serum. Since undesired and excessive myofibroblast formation is a frequent postsurgical complication, we sought culture conditions that would prevent myofibroblast formation. In particular, we analyzed the effect of growth factors, such as epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and platelet-derived growth factor AB (PDGF AB). Our results demonstrate that bFGF is a potent inhibitor of mesodermal differentiation, whereas PDFG AB favours myofibroblast formation and up-regulates expression of TGFbeta receptors I and II. This interesting discovery may help in the prevention and treatment of tissue fibrosis and in particular in the eradication of hypertrophic and keloid scars.
人体中的许多组织微环境，如皮肤，现在被认为含有成体干细胞。在本研究中，我们分析了从机械和酶处理的成人头皮皮肤中分离并扩增的多能人类真皮来源祖细胞群体。这些群体包括巢蛋白阳性和阴性细胞，它们可能成为再生医学中各种治疗应用的便利且丰富的来源。在这里，我们表明，当在含血清的培养基中培养时，这些培养物表现出强烈的分化为中胚层衍生物的倾向，尤其是肌成纤维细胞。由于不期望的和过度的肌成纤维细胞形成是常见的术后并发症，我们寻找能够防止肌成纤维细胞形成的培养条件。特别是，我们分析了生长因子的作用，如表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子AB（PDGF AB）。我们的结果表明，bFGF是中胚层分化的有效抑制剂，而PDGF AB则有利于肌成纤维细胞形成并上调转化生长因子β受体I和II的表达。这一有趣的发现可能有助于预防和治疗组织纤维化，特别是消除增生性瘢痕和瘢痕疙瘩。
