Park Jung-Soon, Lee Chang-Moon, Lee Ki-Young
Department of Material and Biochemical Engineering, Chonnam National University, Gwangju 500-757, South Korea.
Talanta. 2007 Apr 30;72(2):859-62. doi: 10.1016/j.talanta.2006.12.021. Epub 2006 Dec 20.
A self-assembled multilayer (SAMu) including the alginate layer was prepared for detecting Pseudomonas aeruginosa cells in a solution and its potential was evaluated with a BIAcore system. After layer-by-layer formation, the refractive units (RU) values monitored with the biosensor increased by the interaction between the layers. The responses by the binding of P. aeruginosa cells to the alginate-immobilized SAMu were visualized immediately upon injection of the cell suspension. The RU values after injection of the cells were measured with approximately 1152, 656 and 173 for 1x10(9), 1x10(8) and 1x10(7)CFU/ml. This result suggests that the alginate-immobilized SAMu will have useful application for detecting P. aeruginosa cells in a biosensor analysis.
制备了一种包含藻酸盐层的自组装多层膜（SAMu）用于检测溶液中的铜绿假单胞菌细胞，并使用BIAcore系统评估了其潜力。通过逐层形成，生物传感器监测到的折射单位（RU）值因层间相互作用而增加。在注入细胞悬液后，铜绿假单胞菌细胞与固定有藻酸盐的SAMu结合的反应立即显现出来。对于1x10(9)、1x10(8)和1x10(7)CFU/ml的细胞悬液，注入细胞后的RU值分别约为1152、656和173。该结果表明，固定有藻酸盐的SAMu在生物传感器分析中检测铜绿假单胞菌细胞方面将具有有用的应用。
