Rodland Karin D, Adkins Joshua N, Ansong Charles, Chowdhury Saiful, Manes Nathan P, Shi Liang, Yoon Hyunjin, Smith Richard D, Heffron Fred
Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Future Microbiol. 2008 Dec;3(6):625-34. doi: 10.2217/17460913.3.6.625.
Capabilities in mass spectrometry are evolving rapidly, with recent improvements in sensitivity, data analysis and, most important from the standpoint of this review, much higher throughput, allowing analysis of many samples in a single day. This short review describes how these improvements in mass spectrometry can be used to dissect host-pathogen interactions using Salmonella as a model system. This approach has enabled direct identification of the majority of annotated Salmonella proteins, quantitation of expression changes under various in vitro growth conditions and new insights into virulence and expression of Salmonella proteins within host cells. One of the most significant findings is that a relatively high percentage of all the annotated genes (>20%) in Salmonella are regulated post-transcriptionally. In addition, new and unexpected interactions have been identified for several Salmonella virulence regulators that involve protein-protein interactions, suggesting additional functions of these regulators in coordinating virulence expression. Overall high-throughput mass spectrometry provides a new view of host-pathogen interactions, emphasizing the protein products and defining how protein interactions determine the outcome of infection.
质谱分析技术正在迅速发展，最近在灵敏度、数据分析方面有了改进，而从本综述的角度来看，最重要的是通量有了大幅提高，能够在一天内分析多个样本。这篇简短的综述描述了如何利用质谱分析技术的这些改进，以沙门氏菌作为模型系统来剖析宿主-病原体之间的相互作用。这种方法能够直接鉴定出大多数已注释的沙门氏菌蛋白质，定量分析各种体外生长条件下的表达变化，并对宿主细胞内沙门氏菌蛋白质的毒力和表达有了新的认识。最显著的发现之一是，沙门氏菌中所有已注释基因的相当高比例（>20%）是在转录后受到调控的。此外，还发现了几种沙门氏菌毒力调节因子的新的意外相互作用，这些相互作用涉及蛋白质-蛋白质相互作用，表明这些调节因子在协调毒力表达方面具有额外功能。总体而言，高通量质谱分析为宿主-病原体相互作用提供了新的视角，强调了蛋白质产物，并确定了蛋白质相互作用如何决定感染的结果。
