CTX-M型超广谱β-内酰胺酶在韩国一家医院大肠杆菌和肺炎克雷伯菌血流分离株中的传播情况。
Spread of CTX-M-type extended-spectrum beta-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital.
作者信息
Lee Sang-Guk, Jeong Seok Hoon, Lee Hyukmin, Kim Chang Ki, Lee Yangsoon, Koh Eunmi, Chong Yunsop, Lee Kyungwon
机构信息
Department of Laboratory Medicine, Yonsei University College of Medicine, Seodaemun-Gu, Seoul 120-752, South Korea.
出版信息
Diagn Microbiol Infect Dis. 2009 Jan;63(1):76-80. doi: 10.1016/j.diagmicrobio.2008.09.002.
This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum beta-lactamases (ESBLs) in nonduplicate Escherichia coli (n=760) and Klebsiella pneumoniae (n=379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected bla(CTX-M) in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n=32) and K. pneumoniae (n=6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n=22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n=2), CTX-M-65 (n=2), CTX-M-27 (n=1), and CTX-M-32 (n=1) were detected for the 1st time in Korea.
本研究旨在确定2005年1月至2007年10月期间在韩国首尔一家拥有2000张床位的大学医院收集的非重复大肠埃希菌（n = 760）和肺炎克雷伯菌（n = 379）血流分离株中CTX-M型超广谱β-内酰胺酶（ESBLs）的流行情况和特征。采用纸片扩散法和琼脂稀释法测定抗菌药物敏感性。双纸片协同试验在8.7%（66/760）的大肠埃希菌和11.3%（43/379）的肺炎克雷伯菌分离株中检测到ESBLs。聚合酶链反应在66株具有ESBL表型的大肠埃希菌中的60株（90.9%）和43株肺炎克雷伯菌中的9株（20.9%）中检测到bla(CTX-M)。CTX-M-14是大肠埃希菌（n = 32）和肺炎克雷伯菌（n = 6）中最常见的CTX-M型ESBLs。CTX-M-15是大肠埃希菌中第二常见的CTX-M型ESBLs（n = 22），但在肺炎克雷伯菌中未检测到。此外，CTX-M-24（n = 2）、CTX-M-65（n = 2）、CTX-M-27（n = 1）和CTX-M-32（n = 1）在韩国首次被检测到。
Zotero 插件