Hua Jun, Fu Hao, Zhang Rui-Xiu, Chen Dan-Qi, Yan Yang, Chen Fang-Jie, Sun Kai-Lai, Sun Xiu-Ju
Department of Medical Genetics, China Medical University, Shenyang 110001, China.
Yi Chuan. 2008 Dec;30(12):1563-6. doi: 10.3724/sp.j.1005.2008.01563.
S100A4 is an important metastasis-associated gene. Researches have confirmed the close correlation between overexpression of S100A4 gene and gastric cancer's infiltration, lymph node metastasis and in vitro invasiveness of gastric cancer cells. In order to investigate the mechanism of overexpression of S100A4 gene, hypoxia mimetic cobalt chloride (CoCl2) was used to treat gastric cancer cell BGC823, and then the expression of S100A4 mRNA and protein in BGC823 cells were detected by RT-PCR, immunohistochemistry, immunofluorescence, and Western blotting analysis. After treatment with CoCl2, the expression of S100A4 mRNA and protein in BGC823 cell was increased. These results suggested that hypoxia mimetic cobalt chloride could increase the expression of S100A4 gene in gastric cancer cell BGC823.
S100A4是一个重要的转移相关基因。研究已证实S100A4基因的过表达与胃癌的浸润、淋巴结转移以及胃癌细胞的体外侵袭性密切相关。为了探究S100A4基因过表达的机制，使用缺氧模拟物氯化钴（CoCl2）处理胃癌细胞BGC823，然后通过逆转录聚合酶链反应（RT-PCR）、免疫组织化学、免疫荧光和蛋白质免疫印迹分析检测BGC823细胞中S100A4 mRNA和蛋白质的表达。用CoCl2处理后，BGC823细胞中S100A4 mRNA和蛋白质的表达增加。这些结果表明，缺氧模拟物氯化钴可增加胃癌细胞BGC823中S100A4基因的表达。
