Mattocks A R, Croswell S, Jukes R, Huxtable R J
Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, U.K.
Toxicon. 1991;29(4-5):409-15. doi: 10.1016/0041-0101(91)90015-j.
A pneumotoxic pyrrolic metabolite, previously isolated from the bile when rat liver was perfused with the pyrrolizidine alkaloid, monocrotaline, has been identified as 7-glutathionyl-dehydroretronecine. The metabolite showed a TLC spot and HPLC peak corresponding with the latter compound, and a procedure for replacing the thioether group with an ethoxy group converted the metabolite to dehydroretronecine 7-ethyl ether, confirming that the glutathionyl moiety was attached to the 7-position of dehydroretronecine. The same metabolite was detected in bile from rat liver perfused with retrorsine, which is a diester alkaloid similar to monocrotaline, whereas it was not formed from heliotrine, an alkaloid lacking the 7-ester function.
一种先前从用吡咯里西啶生物碱野百合碱灌注大鼠肝脏时的胆汁中分离出的肺毒性吡咯代谢物，已被鉴定为7-谷胱甘肽基脱氢倒千里光裂碱。该代谢物显示出与后一种化合物相对应的薄层色谱斑点和高效液相色谱峰，并且用乙氧基取代硫醚基团的步骤将该代谢物转化为脱氢倒千里光裂碱7-乙醚，证实谷胱甘肽部分连接在脱氢倒千里光裂碱的7位。在用与野百合碱类似的二酯生物碱倒千里光碱灌注大鼠肝脏时的胆汁中检测到了相同的代谢物，而它不是由缺乏7-酯功能的生物碱天芥菜碱形成的。
