亮氨酸响应调节蛋白Lrp通过调控微菌素J25输出蛋白YojI的表达来调节大肠杆菌对微菌素J25的内在抗性。
The leucine-responsive regulatory protein, Lrp, modulates microcin J25 intrinsic resistance in Escherichia coli by regulating expression of the YojI microcin exporter.
作者信息
Socías Sergio B, Vincent Paula A, Salomón Raúl A
机构信息
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas--Universidad Nacional de Tucumán), Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
出版信息
J Bacteriol. 2009 Feb;191(4):1343-8. doi: 10.1128/JB.01074-08. Epub 2008 Dec 12.
Abstract
Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which expels microcin out of cells. Exogenous leucine antagonizes the effect of Lrp, leading to a diminished expression of the pump and an increased susceptibility to microcin J25.
摘要
许多大肠杆菌K-12菌株对肽抗生素微菌素J25表现出内在抗性。在本研究中，我们展示的结果表明，亮氨酸响应调节蛋白Lrp通过作为YojI的正调节因子参与了这一表型，YojI是一种染色体编码的外排泵，可将微菌素排出细胞。外源性亮氨酸拮抗Lrp的作用，导致该泵的表达减少，对微菌素J25的敏感性增加。
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