Meyers Blake C, Axtell Michael J, Bartel Bonnie, Bartel David P, Baulcombe David, Bowman John L, Cao Xiaofeng, Carrington James C, Chen Xuemei, Green Pamela J, Griffiths-Jones Sam, Jacobsen Steven E, Mallory Allison C, Martienssen Robert A, Poethig R Scott, Qi Yijun, Vaucheret Herve, Voinnet Olivier, Watanabe Yuichiro, Weigel Detlef, Zhu Jian-Kang
Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711, USA.
Plant Cell. 2008 Dec;20(12):3186-90. doi: 10.1105/tpc.108.064311. Epub 2008 Dec 12.
MicroRNAs (miRNAs) are approximately 21 nucleotide noncoding RNAs produced by Dicer-catalyzed excision from stem-loop precursors. Many plant miRNAs play critical roles in development, nutrient homeostasis, abiotic stress responses, and pathogen responses via interactions with specific target mRNAs. miRNAs are not the only Dicer-derived small RNAs produced by plants: A substantial amount of the total small RNA abundance and an overwhelming amount of small RNA sequence diversity is contributed by distinct classes of 21- to 24-nucleotide short interfering RNAs. This fact, coupled with the rapidly increasing rate of plant small RNA discovery, demands an increased rigor in miRNA annotations. Herein, we update the specific criteria required for the annotation of plant miRNAs, including experimental and computational data, as well as refinements to standard nomenclature.
微小RNA（miRNA）是由Dicer催化从茎环前体切除产生的约21个核苷酸的非编码RNA。许多植物miRNA通过与特定靶mRNA相互作用，在发育、营养稳态、非生物胁迫反应和病原体反应中发挥关键作用。miRNA不是植物产生的唯一由Dicer衍生的小RNA：21至24个核苷酸的不同类别的短干扰RNA贡献了大量的总小RNA丰度和绝大多数的小RNA序列多样性。这一事实，再加上植物小RNA发现率的迅速增加，要求在miRNA注释方面更加严格。在此，我们更新了植物miRNA注释所需的具体标准，包括实验和计算数据，以及对标准命名法的完善。
