Mizuno Hajime, Tsuyama Naohiro, Date Sachiko, Harada Takanori, Masujima Tsutomu
Graduate School of Biomedical Sciences, Hiroshima University, Japan.
Anal Sci. 2008 Dec;24(12):1525-7. doi: 10.2116/analsci.24.1525.
A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.
通过活单细胞视频质谱法对活的单个细胞进行了直接快速的代谢分析。将细胞质和一个颗粒的内容物吸入纳米电喷雾电离（nano-ESI）尖端，并在添加电离溶剂后通过纳米喷雾直接引入Q-TOF质谱仪。用该方法追踪了培养的大鼠嗜碱性粒细胞白血病细胞系（RBL-2H3）的代谢途径以及色氨酸和组氨酸代谢物的位置。通过t检验对不同位置（如细胞质和颗粒）检测到的峰的t值揭示了每个质谱峰的分子定位。创新了在同步视频显微镜观察下对活细胞进行直接快速的代谢组学分析。
