Ohtsuka Keiichi, Endo Hiroshi, Morimoto Kimio, Vuong Bui N, Ogawa Haruko, Imai Kunitoshi, Takenaka Shigeori
Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan.
Anal Sci. 2008 Dec;24(12):1619-22. doi: 10.2116/analsci.24.1619.
The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.
通过将二抗-碱性磷酸酶偶联物与4-氨基苯磷酸相结合的电化学免疫分析法，尝试检测禽流感病毒（AIV）核蛋白（NP）抗体。释放的4-氨基苯酚通过电化学方法进行定量，水解产物氧化产生的电流在较宽的一抗浓度范围（10 - 1000 ng/ml）内呈线性增加。发现这种电化学免疫分析法对AIV NP抗体的检测限为10 ng/ml。
