Murata T, Ying S Y
Department of Anatomy and Cell Biology, University of Southern California, School of Medicine, Los Angeles 90033.
Life Sci. 1991;49(6):447-53. doi: 10.1016/0024-3205(91)90587-2.
Interleukin-1 beta (IL-1 beta) at doses of 0.15 and 1.5 nM significantly inhibited FSH secretion and stimulated LH secretion by cultured rat pituitary cells after 24-72 hr incubation whereas 15 pM of IL-1 beta was not effective. Treatment with IL-1 beta for 12-48 hr did not affect intracellular content of FSH. However, treatment with 0.15 and 1.5 nM of IL-1 beta for 72 hr significantly suppressed intracellular content of FSH whereas various doses of IL-1 beta incubated with the cells for 12-72 hr showed no effect on the intracellular content of LH. Pretreatment with IL-1 beta for 48 hr inhibited both GnRH-mediated LH and FSH secretions by the pituitary. The secretion of FSH and LH mediated by an activator of protein kinase C, phorbol 12-myristate 13-acetate, was also significantly suppressed by pretreatment with IL-1 beta for 48 hr. These results suggest that (a) IL-1 beta has opposite effects on the secretion of LH and FSH and (b) pretreatment with IL-1 beta suppresses GnRH-mediated stimulation of LH and FSH by the pituitary and this suppressive effect of IL-1 beta may involve the suppression of a protein kinase C-dependent mechanism.
在培养的大鼠垂体细胞中，孵育24 - 72小时后，剂量为0.15和1.5 nM的白细胞介素 - 1β（IL - 1β）显著抑制促卵泡激素（FSH）分泌并刺激促黄体生成素（LH）分泌，而15 pM的IL - 1β则无效。用IL - 1β处理12 - 48小时不影响FSH的细胞内含量。然而，用0.15和1.5 nM的IL - 1β处理72小时显著抑制FSH的细胞内含量，而不同剂量的IL - 1β与细胞孵育12 - 72小时对LH的细胞内含量无影响。用IL - 1β预处理48小时可抑制垂体由促性腺激素释放激素（GnRH）介导的LH和FSH分泌。用蛋白激酶C激活剂佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯介导的FSH和LH分泌，经IL - 1β预处理48小时也显著受到抑制。这些结果表明：（a）IL - 1β对LH和FSH的分泌具有相反的作用；（b）用IL - 1β预处理可抑制垂体由GnRH介导的LH和FSH刺激，且IL - 1β的这种抑制作用可能涉及对蛋白激酶C依赖性机制的抑制。
