Bwanga C O, Hofmo P O, Grevle I S, Einarsson S
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Zentralbl Veterinarmed A. 1991 May;38(4):281-6. doi: 10.1111/j.1439-0442.1991.tb01014.x.
Pooled ejaculates from six fertile boars were frozen under controlled conditions in Teflon FEP-film plastic bags (5 ml) and maxi-straws (2.5 ml) using 3% glycerol as cryoprotectant. The percentages of both post-thaw motility and normal apical ridges were significantly higher (P less than 0.001) for the bags (54.5 and 75%) than for the maxi-straws (40.1 and 59.4%) respectively. For evaluation of the in vivo fertilizing capacity of the frozen-thawed spermatozoa, 26 gilts were inseminated once 24 h after the first observation of standing reflex in their second oestrus, with 5 ml of semen (containing 5 billion spermatozoa) reconstituted in 80 ml of BTS from either bags or maxi-straws. Ova were recovered from the oviducts/uteri 2-4 days following insemination and examined for cleavage and sperm binding to the zona pellucida (ZP). Significantly higher rates (P less than 0.02) of fertilized ova were found in the bag-inseminated (75%) than in maxi-straw inseminated gilts (63%); and similarly their ova had significantly more spermatozoa in the ZP, irrespective of whether they were fertilized or nonfertilized. This study confirmed that the plastic bags are suitable and may be used for packaging single insemination doses of deep frozen boar semen for routine A.I. work.
将来自六头可育公猪的混合射精精液在可控条件下，使用3%甘油作为冷冻保护剂，分别冷冻保存在特氟龙FEP薄膜塑料袋（5毫升）和大细管（2.5毫升）中。解冻后精子活力和正常顶脊的百分比，塑料袋组（分别为54.5%和75%）显著高于大细管组（分别为40.1%和59.4%）（P<0.001）。为评估冻融精子的体内受精能力，在26头后备母猪第二次发情首次观察到静立反射后24小时，用从塑料袋或大细管中取出、重悬于80毫升BTS中的5毫升精液（含50亿精子）对其进行一次授精。授精后2至4天从输卵管/子宫回收卵子，检查卵子的卵裂情况以及精子与透明带（ZP）的结合情况。发现塑料袋授精组的受精卵率（75%）显著高于大细管授精组（63%）（P<0.02）；同样，无论卵子是否受精，塑料袋授精组的卵子在透明带中的精子数量显著更多。本研究证实，塑料袋适用于包装单次授精剂量的深度冷冻公猪精液，可用于常规人工授精工作。
