Hamilton G S, Armstrong D T
Department of Physiology and Obstetrics and Gynaecology, University of Western Ontario, London, Canada.
Biol Reprod. 1991 May;44(5):851-6. doi: 10.1095/biolreprod44.5.851.
The estrous cycles of adult female rats were synchronized with an LHRH agonist on the morning of Day -4 (Day 0 = day of mating). On Day -2, animals received s.c. implants of continuous-infusion osmotic minipumps containing different doses of an FSH preparation (Folltropin) in combination with hCG at various ratios of hCG:FSH or were given single injections of eCG in doses ranging from 15 IU to 60 IU. Rats infused with the optimal dose (3.4 U/day) of FSH ovulated 44.1 +/- 5.4 oocytes/rat while rats treated with the most effective dose (60 IU) of eCG ovulated only 20.5 +/- 4.3 oocytes/rat on the morning of Day 1. The inclusion of hCG in pumps at ratios from 0.188:1 to 0.75:1 (hCG:FSH) had no significant effect on ovulation rate. The importance of synchronization of estrus in successful superovulation was demonstrated by the finding that only 70% of the unsynchronized animals ovulated (29.1 +/- 4.8 oocytes/rat) whereas 95% of the synchronized animals ovulated (51.0 +/- 3.6 oocytes/rat). Oocyte viabilities were assessed by determining fertilization rates and embryonic development in vivo following mating with fertile males. In rats superovulated by use of the FSH regimen, 92% (39.0 +/- 4.1) of the recovered embryos were 1-cell zygotes on Day 1, 89% (36.3 +/- 5.6) were at the 2-cell embryo stage of development on Day 2, and 88% (28.8 +/- 2.2) were at the morula and blastocyst stages on Day 5 following mating on Day 0. The high ovulation rates and oocyte viability in rats receiving infusions of Folltropin following estrus synchronization offer a reliable method for superovulation of adult rats.
在第 -4 天上午，用促黄体生成素释放激素（LHRH）激动剂使成年雌性大鼠的发情周期同步化（第 0 天 = 交配日）。在第 -2 天，动物接受皮下植入含不同剂量促卵泡素（Folltropin）制剂的持续输注渗透微型泵，并以不同的人绒毛膜促性腺激素（hCG）与 FSH 比例联合 hCG，或以 15 国际单位至 60 国际单位的剂量单次注射 eCG。在第 1 天上午，输注最佳剂量（3.4 单位/天）FSH 的大鼠每只排卵 44.1±5.4 个卵母细胞，而用最有效剂量（60 国际单位）eCG 处理的大鼠每只仅排卵 20.5±4.3 个卵母细胞。在微型泵中以 0.188:1 至 0.75:1（hCG:FSH）的比例加入 hCG 对排卵率无显著影响。发情同步化在成功超排卵中的重要性通过以下发现得以证明：未同步化的动物中只有 70%排卵（每只 29.1±4.8 个卵母细胞），而同步化的动物中有 95%排卵（每只 51.0±3.6 个卵母细胞）。通过与可育雄性交配后测定体内受精率和胚胎发育来评估卵母细胞活力。在采用 FSH 方案超排卵的大鼠中，在第 0 天交配后的第 1 天，回收的胚胎中有 92%（39.0±4.1）为单细胞受精卵，第 2 天有 89%（36.3±5.6）处于 2 细胞胚胎发育阶段，第 5 天有 88%（28.8±2.2）处于桑葚胚和囊胚阶段。发情同步化后接受 Folltropin 输注的大鼠具有高排卵率和卵母细胞活力，为成年大鼠超排卵提供了一种可靠方法。
