Enggaard Hansen N, Creutzberg A, Simonsen H B
Department of Fur Animal Production, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Br Vet J. 1991 Mar-Apr;147(2):140-6. doi: 10.1016/0007-1935(91)90104-u.
The time periods and the behavioural pattern of mink euthanized with carbon dioxide (CO2), 100% and 70%, carbon monoxide (CO), 4%, and nitrogen (N2), 100%, are described. The time between the placement of the animal in a glass box and the first symptoms of incoordination (phase I), the period of loss of consciousness (phase II), and, finally, the coma phase until cessation of respiration (phase III) using three groups each of 10 pastel male mink each were recorded. Phase I times ranged from 14 s for CO2, 31 s for N2, and 49 s for CO. In phase II, the difference was even more pronounced, being 5 s for CO2, 15 s for CO, and 45 s for N2. In phase III, the time was 58 s for N2, 134 s and 151 s for CO2 and CO respectively. Hence, the critical period from the time when the animals were placed in the glass box till unconsciousness occurred (phase I and II) was 19 s for CO2, 64 s for CO, and 76 s for N2. The total course of euthanasia was 153 s for CO2, 215 s for CO, and 134 s for N2. The following minimum times will be required before it can be judged to be safe to remove the animals: CO2 and N2: 5 min, and for CO: 6.5 min. In a supplementary experiment, involving a mixture of 70% CO2 and 30% atmospheric air, it was not possible within the allotted time to kill adult male mink.(ABSTRACT TRUNCATED AT 250 WORDS)
描述了用100%和70%的二氧化碳（CO₂）、4%的一氧化碳（CO）和100%的氮气（N₂）对水貂实施安乐死的时间段和行为模式。记录了将动物置于玻璃箱中至出现共济失调的最初症状（第一阶段）、失去意识的时间段（第二阶段），以及最后直至呼吸停止的昏迷阶段（第三阶段），每组使用10只雄性彩色水貂，共三组。第一阶段的时间范围为：CO₂ 14秒、N₂ 31秒、CO 49秒。在第二阶段，差异更为明显，CO₂为5秒、CO为15秒、N₂为45秒。在第三阶段，N₂为58秒，CO₂和CO分别为134秒和151秒。因此，从动物被置于玻璃箱至失去意识（第一阶段和第二阶段）的关键时期，CO₂为19秒、CO为64秒、N₂为76秒。安乐死的总过程，CO₂为153秒、CO为215秒、N₂为134秒。在判定可以安全移走动物之前，需要以下最短时间：CO₂和N₂为5分钟，CO为6.5分钟。在一项补充实验中，使用70% CO₂和30%大气空气的混合物，在规定时间内无法杀死成年雄性水貂。（摘要截短于250字）
