Kowalewski R, Sobolewski K, Malkowski A, Gacko M, Rutkowska I
Department of Vascular Surgery and Transplantology, Medical University of Bialystok, Bialystok, Poland.
Int Angiol. 2008 Dec;27(6):529-35.
Mechanical properties of the vein wall are determined by extracellular matrix components, including glycosaminoglycans (GAGs). The aim of the study was to evaluate the activity of enzymes involved in GAGs degradation pathway in the wall of varicose veins and varicose veins complicated by thrombophlebitis, when compared to the wall of normal ones.
Normal, varicose veins and varicose veins complicated by thrombophlebitis were collected during surgical treatment of 10 patients. Activities of endoglycosidases, sulphatases and exoglycosidases were assessed according to colorimetric methods.
Activities of neutral endoglycosidases degrading chondroitin-4-sulphate (C4S) and heparan sulphate (HS) were decreased, whereas activities of neutral endoglycosidases degrading dermatan sulphate and hyaluronic acid were increased in varicose veins and varicose veins complicated by thrombophlebitis. Activities of acidic endoglycosidases degrading C4S and HS were decreased in varicose veins and varicose veins complicated by thrombophlebitis, whereas activity of acidic endoglycosidases degrading chondroitin-6-sulphate was decreased only in varicose veins complicated by thrombophlebitis. Furthermore increased activities of arylosulphatase B, beta-N-acetylhexosaminidase and alpha-L-iduronidase were demonstrated in varicose veins, as well as in varicose veins complicated by thrombophlebitis.
Changed activities of GAGs-degrading enzymes may contribute to previously reported changes in the content and molecular differentiation of GAGs in the wall of varicose veins that may play a role in the disease pathogenesis.
静脉壁的力学性能由细胞外基质成分决定，包括糖胺聚糖（GAGs）。本研究的目的是评估与正常静脉壁相比，静脉曲张壁以及并发血栓性静脉炎的静脉曲张壁中参与GAGs降解途径的酶的活性。
在对10例患者进行手术治疗期间收集正常静脉、静脉曲张以及并发血栓性静脉炎的静脉曲张。根据比色法评估内切糖苷酶、硫酸酯酶和外切糖苷酶的活性。
在静脉曲张以及并发血栓性静脉炎的静脉曲张中，降解硫酸软骨素-4-硫酸酯（C4S）和硫酸乙酰肝素（HS）的中性内切糖苷酶活性降低，而降解硫酸皮肤素和透明质酸的中性内切糖苷酶活性增加。在静脉曲张以及并发血栓性静脉炎的静脉曲张中，降解C4S和HS的酸性内切糖苷酶活性降低，而仅在并发血栓性静脉炎的静脉曲张中，降解硫酸软骨素-6-硫酸酯的酸性内切糖苷酶活性降低。此外，在静脉曲张以及并发血栓性静脉炎的静脉曲张中，芳基硫酸酯酶B、β-N-乙酰己糖胺酶和α-L-艾杜糖醛酸酶的活性增加。
GAGs降解酶活性的改变可能导致先前报道的静脉曲张壁中GAGs含量和分子分化的变化，这可能在疾病发病机制中起作用。
