Wahid R, Salerno-Gonçalves R, Tacket C O, Levine M M, Sztein M B
Department of Pediatrics, Center for Vaccine Development, University of Maryland, Baltimore, Maryland, USA.
Mucosal Immunol. 2008 Sep;1(5):389-98. doi: 10.1038/mi.2008.30. Epub 2008 Jul 2.
Induction of effective memory T cells is likely to be critical to the level and duration of protection elicited by novel live oral typhoid vaccines. Using cells from volunteers who ingested Salmonella Typhi vaccine strain CVD 909, we characterized the induction of interferon (IFN)-gamma-secreting central (T(CM), CD45RO(+)CD62L(+)) and effector (T(EM), CD45RO(+)CD62L(-)) memory T populations, and their gut-homing potential based on integrin alpha4/beta7 expression. Both CD4(+) T(EM) and T(CM) populations secreted IFN-gamma. However, although CD4(+) T(EM) expressed, or not, integrin alpha(4)/beta(7), CD4(+) T(CM) cells were predominantly integrin alpha(4)/beta(7)(+). In contrast, IFN-gamma-secreting CD8(+) cells were predominantly classical T(EM) and CD45RA(+) T(EM) (T(EMRA), CD45RO(-)CD62L(-)) subsets. However, although CD8(+) T(EM) expressed, or not, integrin alpha(4)/beta(7), CD8(+) T(EMRA) were predominantly integrin alpha(4)/beta(7)(+). This is the first demonstration that oral immunization of humans with S. Typhi elicits diverse IFN-gamma-secreting CD4(+) and CD8(+) T(CM) and T(EM) subsets able to migrate to the gut and other lymphoid tissues.
诱导有效的记忆T细胞可能对新型口服伤寒活疫苗所引发的保护水平和持续时间至关重要。我们使用摄入伤寒沙门氏菌疫苗株CVD 909的志愿者的细胞，对分泌干扰素（IFN）-γ的中枢（T(CM)，CD45RO(+)CD62L(+)）和效应（T(EM)，CD45RO(+)CD62L(-)）记忆T细胞群体的诱导情况及其基于整合素α4/β7表达的肠道归巢潜能进行了表征。CD4(+) T(EM)和T(CM)群体均分泌IFN-γ。然而，尽管CD4(+) T(EM)表达或不表达整合素α(4)/β(7)，但CD4(+) T(CM)细胞主要为整合素α(4)/β(7)(+)。相比之下，分泌IFN-γ的CD8(+)细胞主要是经典的T(EM)和CD45RA(+) T(EM)（T(EMRA)，CD45RO(-)CD62L(-)）亚群。然而，尽管CD8(+) T(EM)表达或不表达整合素α(4)/β(7)，但CD8(+) T(EMRA)主要为整合素α(4)/β(7)(+)。这是首次证明用伤寒沙门氏菌对人类进行口服免疫可引发多种能够迁移至肠道和其他淋巴组织的分泌IFN-γ的CD4(+)和CD8(+) T(CM)及T(EM)亚群。
