[劳斯肉瘤病毒在哺乳动物胚胎组织培养中的合成]
[Synthesis of Rous sarcoma virus in mammalian embryonal tissue cultures].
作者信息
Shevliagin V Ia, Chizhevskaia V I
出版信息
Vopr Onkol. 1976;22(12):54-7.
PMID:190794
Abstract
In embryonal cells of mice and hamsters growing in vitro after Rous virus adsorption on them and long before the onset of cell transformation of the virus was detected in thr form closely associated with cells. After exposure of mammalian cells to actinomycin D or after lowering the temperature of their incubation up to +20 degrees C the cell associated virus was not revealed in embryonal cells. This evidences that the de novo synthesis of Rous virus but not the survival of the virus takes palce in the mentioned cells.
摘要
在劳氏病毒吸附于小鼠和仓鼠的胚胎细胞上之后，且远在检测到病毒导致细胞转化之前，就在体外培养的这些胚胎细胞中发现了与细胞紧密相关形式的病毒。在哺乳动物细胞暴露于放线菌素D之后，或者在将其培养温度降至+20摄氏度之后，在胚胎细胞中未发现与细胞相关的病毒。这证明在上述细胞中发生的是劳氏病毒的从头合成，而非病毒的存活。
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