釉原蛋白介导的磷酸钙的成核与生长。
The nucleation and growth of calcium phosphate by amelogenin.
作者信息
Tarasevich Barbara J, Howard Christopher J, Larson Jenna L, Snead Malcolm L, Simmer James P, Paine Michael, Shaw Wendy J
机构信息
Pacific Northwest National Laboratory, 908 Battelle Blvd., Richland, WA 99354, USA.
出版信息
J Cryst Growth. 2007 Jun 15;304(2):407-415. doi: 10.1016/j.jcrysgro.2007.02.035.
The nucleation processes involved in calcium phosphate formation in tooth enamel are not well understood but are believed to involve proteins in the extracellular matrix. The ability of one enamel protein, amelogenin, to promote the nucleation and growth of calcium phosphate was studied in an in vitro system involving metastable supersaturated solutions. It was found that recombinant amelogenin (rM179 and rp(H)M180) promoted the nucleation of calcium phosphate compared to solutions without protein. The amount of calcium phosphate increased with increasing supersaturation of the solutions and increasing protein concentrations up to 6.5 μg/mL. At higher protein concentrations, the amount of calcium phosphate decreased. The kinetics of nucleation was studied in situ and in real time using a quartz crystal microbalance (QCM) and showed that the protein reduced the induction time for nucleation compared to solutions without protein. This work shows a nucleation role for amelogenin in vitro which may be promoted by the association of amelogenin into nanosphere templates, exposing charged functionality at the surface.
牙釉质中磷酸钙形成所涉及的成核过程尚未完全明确，但据信与细胞外基质中的蛋白质有关。在一个涉及亚稳态过饱和溶液的体外系统中，研究了一种牙釉质蛋白——釉原蛋白促进磷酸钙成核和生长的能力。研究发现，与不含蛋白质的溶液相比，重组釉原蛋白（rM179和rp(H)M180）能促进磷酸钙的成核。随着溶液过饱和度的增加以及蛋白质浓度增加至6.5μg/mL，磷酸钙的量也随之增加。在更高的蛋白质浓度下，磷酸钙的量减少。使用石英晶体微天平（QCM）对成核动力学进行了原位实时研究，结果表明，与不含蛋白质的溶液相比，该蛋白质缩短了成核的诱导时间。这项工作表明了釉原蛋白在体外具有成核作用，这可能是由釉原蛋白缔合形成纳米球模板，使表面带有带电官能团所促进的。
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