Li Jie, Li Bao-lan, Zhang Hai-qing, Xu Shao-fa, Liu Zhi-dong, Yue Wen-tao, Han Yi
Combination Department, Beijing Chest Hospital, Beijing 101149, China.
Zhonghua Yi Xue Za Zhi. 2008 Nov 18;88(42):2982-5.
To investigate the relationship between vascular endothelial growth factor C (VEGF-C) expression level and lymph node metastasis in non small cell lung cancer (NSCLC).
Fifty-two NSCLC patients, 38 males and 14 females, aged (59+/-11) (29-77), were divided into 2 groups based on the pathological examination: lymph node metastasis positive group (n=25) and lymph node metastasis negative group (n=27). RT-PCR and immunohistochemistry were used to detect the mRNA and protein expression of VEGF-C in the tumor tissues and lymph nodes resected during operation.
The VEGF-C mRNA expression level in the lung tumor tissue of the lymph node metastasis positive group was 0.273+/-0.179, significantly higher than that in the lymph node metastasis negative group (0.089+/-0.087, P<0.01). The VEGF-C mRNA expression level in the positive lymph node of the lymph node metastasis positive group was 0.207+/-0.174, significantly higher than that in the lymph node metastasis negative group (0.114+/-0.107, P<0.01), but not significantly different from that in the negative lymph nodes of the same group(0.196+/-0.186, P>0.05). The VEGF-C protein positive rate of the lung cancer tissues of the lymph node metastasis positive group was 93.3% (14/15), significantly higher than that of the lymph node metastasis negative group (6.7%, 1/15, P<0.01). The VEGF-C protein positive rate of the metastasis positive lymph noses was 80.4% (37/46), and all 52 metastasis negative lymph nodes were VEGF-C protein negative.
VEGF-C mRNA and protein expression levels predict lymph node metastasis and can be useful predictors of lymph node metastasis in NSCLC.
探讨血管内皮生长因子C（VEGF-C）表达水平与非小细胞肺癌（NSCLC）淋巴结转移的关系。
52例NSCLC患者，男38例，女14例，年龄（59±11）岁（29 - 77岁），根据病理检查分为2组：淋巴结转移阳性组（n = 25）和淋巴结转移阴性组（n = 27）。采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法检测手术切除的肿瘤组织及淋巴结中VEGF-C的mRNA和蛋白表达。
淋巴结转移阳性组肺肿瘤组织中VEGF-C mRNA表达水平为0.273±0.179，显著高于淋巴结转移阴性组（0.089±0.087，P < 0.01）。淋巴结转移阳性组阳性淋巴结中VEGF-C mRNA表达水平为0.207±0.174，显著高于淋巴结转移阴性组（0.114±0.107，P < 0.01），但与同组阴性淋巴结（0.196±0.186，P > 0.05）相比差异无统计学意义。淋巴结转移阳性组肺癌组织中VEGF-C蛋白阳性率为93.3%（14/15），显著高于淋巴结转移阴性组（6.7%，1/15，P < 0.01）。转移阳性淋巴结中VEGF-C蛋白阳性率为80.4%（37/46），52枚转移阴性淋巴结VEGF-C蛋白均为阴性。
VEGF-C mRNA和蛋白表达水平可预测NSCLC的淋巴结转移，是NSCLC淋巴结转移的有用预测指标。
