中国重庆儿童人偏肺病毒感染的高血清阳性率。
High seroprevalence of human metapneumovirus infection in children in Chongqing, China.
作者信息
Zhang Qin, Yang Xi-qiang, Zhao Yao, Zhao Xiao-dong
机构信息
Division of Immunology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
出版信息
Chin Med J (Engl). 2008 Nov 5;121(21):2162-6.
BACKGROUND
The human metapneumovirus (hMPV) is a newly discovered respiratory viral pathogen that was first isolated in 2001 in the Netherlands. Its global distribution and long history of infection in humans have been well documented. In this study, we assessed the seropositivity of hMPV IgG antibodies in children in Chongqing, China.
METHODS
The specificity of the enzyme linked immunosorbent assay (ELISA) was first validated by using respiratory syncytial virus (RSV) infected, antigen subtracted reference serum and by performing western blotting using anti-hMPV animal serum. This assay was used to determine the presence of the IgG antibody against hMPV and RSV in 325 serum samples obtained from children aged 0 - 6 years.
RESULTS
No crossreaction was detected by ELISA between the antibodies to hMPV and RSV. The seropositivity of the anti-hMPV IgG antibody was 74.5% in children aged 0 to 5 months, 64.0% in 6 to 11 months, 72.7% in 12 to 23 months, 87.1% in 24 to 35 months and 90.3% in 3 to 6 years.
CONCLUSIONS
hMPV is a common and significant respiratory pathogen in Chinese children. Almost all individuals are exposed to hMPV by age 6 years.
背景
人偏肺病毒（hMPV）是一种新发现的呼吸道病毒病原体，于2001年首次在荷兰分离出来。其全球分布以及在人类中的长期感染历史已有充分记录。在本研究中，我们评估了中国重庆儿童中hMPV IgG抗体的血清阳性率。
方法
首先通过使用呼吸道合胞病毒（RSV）感染的、去除抗原的参考血清以及使用抗hMPV动物血清进行蛋白质印迹法来验证酶联免疫吸附测定（ELISA）的特异性。该测定用于确定从0至6岁儿童获得的325份血清样本中抗hMPV和RSV IgG抗体的存在情况。
结果
ELISA检测未发现抗hMPV和RSV抗体之间存在交叉反应。抗hMPV IgG抗体的血清阳性率在0至5个月的儿童中为74.5%，6至11个月为64.0%，12至23个月为72.7%，24至35个月为87.1%，3至6岁为90.3%。
结论
hMPV是中国儿童中一种常见且重要的呼吸道病原体。几乎所有个体在6岁时都曾接触过hMPV。
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