[百草枯中毒致大鼠肺损伤中JNK和c-Jun的变化]

[Change of JNK and c-Jun in lung injury associated with paraquat poisoning of rats].

作者信息

Liu Jian-Hui, Sun Zhi-Ping, Ma Yu-Teng

机构信息

Hehei General Hospital, Shijiazhuang 050051, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Jul;26(7):406-9.

PMID:19080378

Abstract

OBJECTIVE

To investigate the change of JNK and c-Jun in lung injury associated with paraquat poisoning of rats.

METHODS

46 Rats were randomly divided into four groups: PQ group (n = 12), control group (n = 10), PQ + ZnPP group (n = 12) and PQ + Hm group (n = 12). The rats were injected with 2% PQ (25 mg/kg, ip) in PQ group. ZnPP and Hemin (10 mg/kg, 10 mg/ml) were injected through inguinal vein before intraperitoneal administration of 2% paraquat in PQ + ZnPP group and PQ + Hm group respectively. The rats were injected NS (1 ml/kg, ip) in control group. HE dyeing of lung tissue and MDA content of plasma were used for estimating the injury of lung tissue. The content of CO in the lung tissue was determined. The expression of HO-1 mRNA of the lung tissue was detected by the reverse transcription-polymerase chain reaction. The phosphorylation of JNK and c-Jun was evaluated by Western blot analysis.

RESULTS

The degree of lung injury in PQ group and PQ + ZnPP group was higher than that in control group and PQ + Hm group. But in PQ + Hm group the degree of lung injury was lower. The content of MDA in PQ group and PQ + ZnPP group was higher than that in control group and PQ + Hm group (P < 0.01). The content of MDA in PQ + Hm group was higher than that in control group (P < 0.05). The content of CO in lung tissue in PQ group, PQ + ZnPP group and PQ + Hm group was and (1.08 +/- 0.15 mg/L) respectively, and higher than that in control group (P < 0.01). The content of CO in lung tissue in PQ + Hm group was significantly higher than that in PQ + ZnPP group (P < 0.01). The expression of HO-1 and the phosphorylation of JNK (55.24 +/- 9.34, 38.15 +/- 10.71, 128.55 +/- 19.43) and c-Jun (23.16 +/- 4.85, 15.49 +/- 3.13, 44.89 +/- 10.37) were increased remarkably in PQ group, PQ + ZnPP group and PQ + Hm group. Those in PQ + Hm group were higher significantly than PQ group and PQ + ZnPP group (P < 0.01). Those in PQ + ZnPP group were lower than PQ group (P < 0.05).

CONCLUSION

The increase of CO of lung tissue in rats at the lung injury associated with paraquat poisoning reduces the acute lung injury of rats. The level of JNK and c-Jun phosphorylation increases obviously, especially after Hemin is utilized.

摘要

目的

探讨大鼠百草枯中毒致肺损伤时JNK和c-Jun的变化。

方法

46只大鼠随机分为四组：百草枯组（n = 12）、对照组（n = 10）、百草枯+锌原卟啉组（n = 12）和百草枯+血红素组（n = 12）。百草枯组大鼠腹腔注射2%百草枯（25 mg/kg）。百草枯+锌原卟啉组和百草枯+血红素组分别于腹腔注射2%百草枯前经腹股沟静脉注射锌原卟啉和血红素（10 mg/kg，10 mg/ml）。对照组大鼠腹腔注射生理盐水（1 ml/kg）。采用肺组织HE染色及血浆丙二醛含量评估肺组织损伤情况。测定肺组织中一氧化碳含量。采用逆转录-聚合酶链反应检测肺组织HO-1 mRNA表达。通过蛋白质免疫印迹分析评估JNK和c-Jun的磷酸化水平。

结果

百草枯组和百草枯+锌原卟啉组肺损伤程度高于对照组和百草枯+血红素组。但百草枯+血红素组肺损伤程度较低。百草枯组和百草枯+锌原卟啉组丙二醛含量高于对照组和百草枯+血红素组（P < 0.01）。百草枯+血红素组丙二醛含量高于对照组（P < 0.05）。百草枯组、百草枯+锌原卟啉组和百草枯+血红素组肺组织一氧化碳含量分别为（1.08±0.15 mg/L），均高于对照组（P < 0.01）。百草枯+血红素组肺组织一氧化碳含量显著高于百草枯+锌原卟啉组（P < 0.01）。百草枯组、百草枯+锌原卟啉组和百草枯+血红素组HO-1表达及JNK（55.24±9.34，38.15±10.71，128.55±19.43）和c-Jun（23.16±4.85，15.49±3.13，44.89±10.37）磷酸化水平显著升高。百草枯+血红素组显著高于百草枯组和百草枯+锌原卟啉组（P < 0.01）。百草枯+锌原卟啉组低于百草枯组（P < 0.05）。