He Xiao-yan, Sun Qi, Li Zhong-wang, Lu Zhong-qiu, Hong Guang-liang, Liang Huan, Qiu Qiao-meng, Hu Guo-xin
Emergercy Medical Department, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Mar;27(3):149-52.
To investigate the expression of inflammatory factors in lung tissue of acute paraquat (PQ) poisoned rats.
Fifty male SD rats were randomized divided into two groups: the normal control group (NC group, n = 10) and the PQ group (n = 40). The 1 ml saline was administered once in normal control group. The PQ group was administered with 20 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced lung injury. At six hours, at the first, the third and the seventh day the PQ group were sacrificed, while at the first day the normal control group was sacrificed. The level of tumor necrosis factor alpha (TNF-alpha) mRNA, interleukin 10 (IL-10) mRNA, high mobility group box 1 (HMGB-1) mRNA in lung of rats were detected. Meanwhile, pathological changes of the lung were examined under optical microscope.
Compared with that in normal control group, TNF-alpha mRNA expression in lung tissue of PQ group reached the peak at the six hour and decreased slowly at the first day [(0.740 +/- 0.100) and (0.584 +/- 0.049) respectively]. At the six hour and the first day in PQ group it was significantly higher than that in normal control group (P < 0.05 or P < 0.01). IL-10 mRNA expression in lung tissue of PQ group was elevated at the six hour, reached the peak at the first day, at the third day [(0.551 +/- 0.016) and (0.524 +/- 0.010) respectively] and the seventh day also higher than that in normal control group. At the first and the seventh day in the PQ group it was significantly higher than that in normal control group (P < 0.01). Meanwhile, HMGB-1 mRNA expression in lung tissue of PQ group was also elevated at the six hour, reached the peak at the first day, at the third [(0.695 +/- 0.060), (0.871 +/- 0.154) and (0.819 +/- 0.188) respectively] and the seventh day also higher than that in normal control group. At six hour, the first and the third day in the PQ group it was significantly higher than that in normal control group (P < 0.01). The histological changes such as alveolar edema, hemorrhage and inflammatory cell infiltration in the PQ group were more than those in the normal control group.
In rats after PQ intoxication the levels of the inflammatory factors TNF-alpha, IL-10 and HMGB-1 are higher than normal rats, and inflammatory could play an important role in lung injury of poisoned rats.
探讨急性百草枯（PQ）中毒大鼠肺组织中炎症因子的表达情况。
将50只雄性SD大鼠随机分为两组：正常对照组（NC组，n = 10）和PQ组（n = 40）。正常对照组腹腔注射1 ml生理盐水1次。PQ组腹腔注射20 mg/kg 1%的PQ建立PQ诱导的肺损伤模型。在6小时、第1天、第3天和第7天处死PQ组大鼠，第1天处死正常对照组大鼠。检测大鼠肺组织中肿瘤坏死因子α（TNF-α）mRNA、白细胞介素10（IL-10）mRNA、高迁移率族蛋白B1（HMGB-1）mRNA的水平。同时，在光学显微镜下观察肺组织的病理变化。
与正常对照组相比，PQ组肺组织中TNF-α mRNA表达在6小时达到峰值，第1天缓慢下降[分别为(0.740±0.100)和(0.584±0.049)]。PQ组在6小时和第1天显著高于正常对照组（P < 0.05或P < 0.01）。PQ组肺组织中IL-10 mRNA表达在6小时升高，第1天达到峰值，第3天[(0.551±0.016)和(0.524±0.010)]及第7天也高于正常对照组。PQ组在第1天和第7天显著高于正常对照组（P < 0.01）。同时，PQ组肺组织中HMGB-1 mRNA表达在6小时也升高，第1天达到峰值，第3天[(0.695±0.060)、(0.871±0.154)和(0.819±0.188)]及第7天也高于正常对照组。PQ组在6小时、第1天和第3天显著高于正常对照组（P < 0.01）。PQ组的组织学变化如肺泡水肿、出血和炎症细胞浸润比正常对照组更明显。
PQ中毒大鼠炎症因子TNF-α、IL-10和HMGB-1水平高于正常大鼠，炎症在中毒大鼠肺损伤中可能起重要作用。
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