富血小板血浆对海绵体神经再生的影响：大鼠实验

[Effect of platelet rich plasma on the regeneration of cavernous nerve: experiment with rats].

作者信息

Ding Xie-gang, Li Shi-wen, Zheng Xin-min, Hu Li-quan, Hu Wan-li, Luo Yi

机构信息

Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2008 Sep 23;88(36):2578-80.

PMID:19080657

Abstract

OBJECTIVE

To investigate the effect of platelet rich plasma (PRP) on the regeneration of injured cavernous nerve (CN).

METHODS

Blood was collected from the hearts of 6 SD rats to prepare PRP. 24 male adult rats were randomly divided into 3 equal groups: pure suture group undergoing bilateral CN transaction and pure suture immediately, PRP group undergoing bilateral CN transaction + suture + PRP 200 microl to the site of suture, and sham operation group. 3 months later intracavernous pressure (ICP) was measured by CN electrostimulation and then samples of CN were obtained to undergo pathological examination.

RESULTS

3 months later after surgery, the ICP of the pure suture group was (46 +/- 8) cm H2O, significantly lower than that of the sham group [(109 +/- 13) cm H2O, P < 0.01], and that of the PRP group was (94 +/- 13) cm H2O, significantly higher than that of the pure sutured group (P < 0.01), however, still significantly lower than that of the sham operation group (P < 0.05). The number of axons of CN in the PRP group was (121 +/- 16), significantly higher than that of the pure sutured group (70 +/- 14, P < 0.01); however, still significantly lower than that of the sham operation group (181 +/- 21, P < 0.01).

CONCLUSION

PRP can promote the regeneration of injured CN and the recovery of erectile function.

摘要

目的

探讨富血小板血浆（PRP）对损伤海绵体神经（CN）再生的影响。

方法

从6只SD大鼠心脏取血制备PRP。将24只成年雄性大鼠随机分为3组，每组8只：单纯缝合组，双侧CN离断后立即单纯缝合；PRP组，双侧CN离断+缝合+在缝合部位注射200微升PRP；假手术组。3个月后通过CN电刺激测量海绵体内压（ICP），然后获取CN样本进行病理检查。

结果

术后3个月，单纯缝合组的ICP为（46±8）cm H₂O，显著低于假手术组[（109±13）cm H₂O，P<0.01]；PRP组的ICP为（94±13）cm H₂O，显著高于单纯缝合组（P<0.01），但仍显著低于假手术组（P<0.05）。PRP组CN的轴突数量为（121±16），显著高于单纯缝合组（70±14，P<0.01）；但仍显著低于假手术组（181±21，P<0.01）。