Herrmann V W, Reuter W, Starke I
Z Gesamte Inn Med. 1976 Dec 1;31(23):988-91.
By means of cholesterol feeding on rabbits the change of the beta- and pre-beta-lipoprotein relations is observed by densitometric evaluation of lipoprotein electrophoreses on cellulose acetate foils and in agar-agarose gel. At the same time the changes of the cholesterol and triglyceride content of the sera are determined. The agar-agarose-gel electrophoreses prove to be more suitable for the densitometric evaluation compared with the electrophoreses on cellulose acetate foils, since they give more exact results. A reason for this is the worse demarcability of the pre-beta-lipoprotein proportion in the curves of the densitometre of acetate foils. The percental distributions of lipoprotein in patients of different types of HLP are determined and with the help of the results the evidence of the visual judgment of the lipoprotein electrophoreses is discussed.
通过给兔子喂食胆固醇,借助对醋酸纤维素箔片和琼脂 - 琼脂糖凝胶上脂蛋白电泳的光密度评估,观察β脂蛋白和前β脂蛋白关系的变化。同时测定血清中胆固醇和甘油三酯含量。与醋酸纤维素箔片上的电泳相比,琼脂 - 琼脂糖凝胶电泳被证明更适合光密度评估,因为其结果更准确。原因是醋酸纤维素箔片光密度计曲线中前β脂蛋白比例的分界性较差。确定了不同类型高脂蛋白血症(HLP)患者中脂蛋白的百分比分布,并借助这些结果讨论了脂蛋白电泳视觉判断的证据。