Lerma-García M J, Ramis-Ramos G, Herrero-Martínez J M, Gimeno-Adelantado J V, Simó-Alfonso E F
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, 46100 Burjassot, Valencia, Spain.
J Chromatogr A. 2009 Jan 9;1216(2):230-6. doi: 10.1016/j.chroma.2008.11.056. Epub 2008 Nov 27.
Aliphatic and triterpene alcohols present in vegetable oils have been identified and determined by HPLC using UV-vis and MS detection after previous derivatization with diphenic anhydride. The alcoholic fraction was obtained by saponification, extraction and TLC (according to the European Union official procedure). Derivatization was performed in tetrahydrofuran in the presence of suspended grinded urea, which increases the reaction rate and yield. Derivatized extracts were chromatographed on a C8 column using gradient elution with acetonitrile/water mixtures containing 0.1% acetic acid, with UV-vis followed by negative-ion mode MS detection. Using linear discriminant analysis of the HPLC-MS data (extracted ion chromatograms), oil samples belonging to seven botanical origins (hazelnut, sunflower, corn, extra virgin olive, soybean, peanut and grapeseed) were correctly classified with excellent resolution among all the categories.
植物油中存在的脂肪族和三萜醇，在先前用联苯二甲酸酐衍生化后，通过高效液相色谱法（HPLC）并使用紫外可见光谱（UV-vis）和质谱（MS）检测进行了鉴定和测定。醇馏分通过皂化、萃取和薄层色谱法（根据欧盟官方程序）获得。衍生化在四氢呋喃中，在悬浮研磨的尿素存在下进行，这会提高反应速率和产率。衍生化提取物在C8柱上进行色谱分离，使用含0.1%乙酸的乙腈/水混合物进行梯度洗脱，先进行紫外可见光谱检测，然后进行负离子模式质谱检测。通过对HPLC-MS数据（提取离子色谱图）进行线性判别分析，属于七个植物来源（榛子、向日葵、玉米、特级初榨橄榄油、大豆、花生和葡萄籽油）的油样在所有类别中都以优异的分辨率被正确分类。
